基质细胞衍生因子1(SDF1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Stromal Cell Derived Factor 1 (SDF1)
CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12
- 编号SEA122Mu
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围62.5-4,000pg/mL
- 灵敏度最小可检测剂量小于等于26.1pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 2722¥ 3888¥ 17496¥ 33048¥ 272160
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
本试剂盒用于检测基质细胞衍生因子1(SDF1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的基质细胞衍生因子1(SDF1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 82-97 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的基质细胞衍生因子1(SDF1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中基质细胞衍生因子1(SDF1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-88% | 91-102% | 88-95% | 83-101% |
| EDTA plasma(n=5) | 81-93% | 97-104% | 96-103% | 85-94% |
| heparin plasma(n=5) | 95-105% | 82-101% | 95-105% | 78-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将基质细胞衍生因子1(SDF1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的基质细胞衍生因子1(SDF1)与连接于固相载体上的抗体结合，然后加入生物素化的基质细胞衍生因子1(SDF1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的基质细胞衍生因子1(SDF1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA122Mu02 | 基质细胞衍生因子1(SDF1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA122Mu01 | 基质细胞衍生因子1(SDF1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA122Mu02 | 基质细胞衍生因子1(SDF1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA122Mu01 | 基质细胞衍生因子1(SDF1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA122Mu71 | 基质细胞衍生因子1(SDF1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA122Mu81 | 基质细胞衍生因子1(SDF1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MEA122Mu | 基质细胞衍生因子1(SDF1)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA122Mu | 基质细胞衍生因子1(SDF1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA122Mu | 基质细胞衍生因子1(SDF1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Critical Care | Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD)[Goescholar: source] |
| 22 | The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vessels[ScienceDirect: S0142961209012654] |
| 22 | The use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanisms[ScienceDirect: S0142961211011422] |
| Phytomedicine | Tanshinone IIA increases recruitment of bone marrow mesenchymal stem cells to infarct region via up-regulating stromal cell-derived factor-1/CXC chemokine receptor 4 axis in a myocardial ischemia model[ScienceDirect: S0944711310003260] |
| Graefe's Archive for Clinical and Experimental Ophthalmology | Monocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cells[PubMed: 21655971] |
| Molecular Therapy | Pleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemia[PubMed: 21224834] |
| Oncogene | Elevated PLGF contributes to small-cell lung cancer brain metastasis[PubMed: 22797069] |
| International Journal of Nephrology | Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis[PubMed: 22792461] |
| Rheumatology International | Serum levels of selected chemokines in systemic lupus erythematosus patients[Pubmed: 22461186] |
| Experimental Eye Research | Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cells[Pubmed: 24780853] |
| Atherosclerosis | The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trial[Pubmed:24530788] |
| International Journal of Cardiology | Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men[Pubmed:24286591] |
| Tissue Engineering Part A | Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat[Pubmed:24814095] |
| Pharmacol Rep.? | Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression[Pubmed:25150002] |
| Journal of Stroke and Cerebrovascular Diseases | Circulating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke Patients[Pubmed:25444027] |
| Pharmacological Reports | The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders[Pubmed:25560580] |
| In Vitro Cellular & Developmental Biology - Animal | Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells[Pubmed:25636237] |
| Pharmacological Reports | Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder[PubMed: 26481549] |
| Biochem Biophys Res Commun | Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats[PubMed: 26392311] |
| Am J Transl Res | Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats[PubMed: 26396666] |
| Medicine (Baltimore) | A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural Effusion[PubMed: 26632727] |
| Pharmacological Reports | A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity[Pubmed:27208878] |
| Frontiers in Psychiatry | Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity.[pubmed:28149283] |
| European Journal of Applied Physiology | High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cellsand depressed shedding of vascular endothelial cells undergoing hypoxia.[pubmed:27761657] |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | AMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungs[pubmed:29233725] |
| International Journal of Clinical and Experimental Pathology | Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens[ISSN:1936-2625/IJCEP0058424] |
| BioMed Research International. | c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancer[pubmed:28573141] |
| Scientific Reports | Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms.[pubmed:28842629] |
| Biomaterials | Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study.[pubmed:29017079] |
| Biomaterials Science | The effects of Sr-incorporated micro/nano rough titanium surface on rBMSC migration and osteogenic differentiation for rapid osteointegration[Pubmed:29850672] |
| International Journal of Molecular Medicine | Low‑intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1‑mediated SDF‑1 expression[Pubmed:29620151] |
| Cardiovascular Drugs and Therapy | β‐blockers interfere with cell homing receptors and regulatory proteins in a model of spontaneously hypertensive rats[Pubmed:29752864] |
| Cytotherapy | Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation[Doi: 10.1016/j.jcyt.2018.07.007] |
| ACS Applied Materials & Interfaces | Functionalization of Silk Fibroin Electrospun Scaffolds via BMSC Affinity Peptide Grafting through Oxidative Self-Polymerization of Dopamine for Bone Regeneration[Pubmed: 30777748] |
| World Journal of Stem Cells | Stromal cell-derived factor-1α promotes recruitment and differentiation of nucleus pulposus-derived stem cells[Pubmed: 30949297] |
| Stem Cells International | Effects of Stromal Cell-Derived Factor-1α Secreted in Degenerative Intervertebral Disc on Activation and Recruitment of Nucleus Pulposus-Derived Stem Cells[Pubmed: 31827537] |
| Stem Cell Res Ther | Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus[Pubmed: 32894200] |
| Journal of tissue engineering and regenerative medicine | Enhanced recruitment and hematopoietic reconstitution of bone marrow‐derived mesenchymal stem cells in bone marrow failure by the SDF‐1/CXCR4[Pubmed: 32633015] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia[33878473] |
| pharmaceuticals | bFGF and SDF-1¦Á Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts[33800631] |
| Microb Biotechnol | Combination of an engineered Lactococcus lactis expressing CXCL12 with light‐emitting diode yellow light as a treatment for scalded skin in mice[34310856] |
