正五聚蛋白3(PTX3)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Pentraxin 3, Long (PTX3)
TNFAIP5; TSG-14; Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein
- 编号SEK411Mu
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.156-10ng/mL
- 灵敏度最小可检测剂量小于等于0.059ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
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产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测正五聚蛋白3(PTX3)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的正五聚蛋白3(PTX3)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 86-94 | 89 |
| heparin plasma(n=5) | 97-104 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的正五聚蛋白3(PTX3)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中正五聚蛋白3(PTX3)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-91% | 88-102% | 80-97% | 81-97% |
| EDTA plasma(n=5) | 86-94% | 78-104% | 83-103% | 78-93% |
| heparin plasma(n=5) | 78-91% | 99-105% | 78-95% | 93-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将正五聚蛋白3(PTX3)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的正五聚蛋白3(PTX3)与连接于固相载体上的抗体结合，然后加入生物素化的正五聚蛋白3(PTX3)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的正五聚蛋白3(PTX3)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPK411Mu01 | 正五聚蛋白3(PTX3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAK411Mu01 | 正五聚蛋白3(PTX3)多克隆抗体 | WB; IHC; ICC; IP. |
| SEK411Mu | 正五聚蛋白3(PTX3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMK411Mu | 正五聚蛋白3(PTX3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Mediators of Inflammation | Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model[PubMed: 22966213] |
| Ibnosina Journal of Medicine and Biomedical Sciences | Effect of Vagus Nerve Stimulation on Focal Transient Cerebral Ischemia and Reperfusion in Adult Male White New Zealand Rabbits[Sfu: Source] |
| Hemodialysis International | Hemodialysis-induced changes in the blood composition affect function of the endothelium[Onlinelibrary: hdi.12148] |
| Hemodial Int. | Hemodialysis‐induced changes in the blood composition affect function of the endothelium[Pubmed:24612443] |
| Journal of Obstetrics and Gynaecology Research | The effects of sunitinib on endometriosis[Pubmed:25093747] |
| Gynecol Endocrinol. | Exenatide improves ovarian and endometrial injury and preserves ovarian reserve in streptozocin induced diabetic rats[Pubmed:25366063] |
| Taiwanese Journal of Obstetrics and Gynecology | Ovarian failure in diabetic rat model: Nuclear factor-kappaB, oxidative stress, and pentraxin-3[Pubmed:25510691] |
| Journal of Obstetrics and Gynaecology Research | Octreotide protects ovary against ischemia–reperfusion injury in rats: Evaluation of histological and biochemical parameters[PubMed: 26223286] |
| J Obstet Gynaecol | The long pentraxin-3 is a useful marker for diagnosis of ovarian torsion: An experimental rat model[PubMed: 26470829] |
| Neurology Research International | Accumulation of α-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers[5952149] |
| Journal of Diabetes Research | Study on Association of Pentraxin 3 and Diabetic Nephropathy in a Rat Model[Pubmed:29725602] |
| World Journal of Cardiovascular Diseases | The Relationship between Serum Pentraxin-3 Levels and Severity of Coronary Heart Disease[paperID:86206] |
| Advances in Medical Sciences | Serum levels and gene expression of pentraxin 3 are elevated in COPD[Pubmed: 30572222] |
| BioMed Research International | Pentraxin 3 Detects Clinically Significant Fibrosis in Patients with Chronic Viral Hepatitis C[] |
| Evidence-Based Complementary and Alternative Medicine | Jie Du Tong Ye San Prevents N-Nitrosomethylbenzylamine-Induced Esophageal Carcinogenesis via Inhibition of Inflammation and Proliferation[] |
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| Scientific Reports | The role of CRP and Pentraxin 3 in the prediction of systemic inflammatory response syndrome and death in acute pancreatitis[Pubmed: 31798002] |
| J Obstet Gynaecol | Losartan ameliorates ovarian ischaemia/reperfusion injury in rats: an experimental study[Pubmed: 31955629] |
| J Pediatr Adolesc Gynecol | Effects of Methylene Blue on Ovarian TorsionLDetorsion Injury in a Rat Model[Pubmed: 32593749] |
| Acta Cirurgica Brasileira | Therapeutic effects of vitamin D on acetic acid-induced colitis in rats[Pubmed: 32555936] |
| Acta Cardiol Sin | Pentraxin 3 as a marker for cardiovascular disease risk in overweight and obese children[33716459] |
| International Journal of Endocrinology | Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡[34035808] |
| Nutrients | High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese …[34684637] |
| J Obstet Gynaecol Res | Protective effects of dichloroacetic acid on endometrial injury and ovarian reserve in an experimental rat model of diabetes mellitus[34595798] |
