抗增殖细胞核抗原(PCNA)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Proliferating Cell Nuclear Antigen (PCNA)
Cyclin
- 编号SEA591Mi
- 物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.156-10ng/mL
- 灵敏度最小可检测剂量小于等于0.055ng/mL.
- 样本类型Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 2722¥ 3888¥ 17496¥ 33048¥ 272160
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产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒对检测抗增殖细胞核抗原(PCNA)具有较高的灵敏度。
经检测人、小鼠、大鼠之间有100%的交叉反映。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将抗增殖细胞核抗原(PCNA)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的抗增殖细胞核抗原(PCNA)与连接于固相载体上的抗体结合，然后加入生物素化的抗增殖细胞核抗原(PCNA)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗增殖细胞核抗原(PCNA)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA591Mi01 | 抗增殖细胞核抗原(PCNA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA591Mi01 | 抗增殖细胞核抗原(PCNA)多克隆抗体 | WB; IHC |
| MAA591Mi22 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC |
| MAA591Mi23 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC |
| CAA591Mi22 | 抗抗增殖细胞核抗原(PCNA)单克隆抗体 | WB |
| MAA591Mi24 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA591Mi21 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC; ICC/IF |
| SEA591Mi | 抗增殖细胞核抗原(PCNA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA591Mi | 抗增殖细胞核抗原(PCNA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
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