武汉云克隆科技股份有限公司

血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Angiotensin I Converting Enzyme 2 (ACE2)

ACEH; ACEII; ACE-II; Peptidyl-Dipeptidase A; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15

  • 血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法)实验结果图
  • SEB886Ra.jpg标准曲线图
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管紧张素转化酶2(ACE2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管紧张素转化酶2(ACE2)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
serum(n=5)99-105102
EDTA plasma(n=5)79-9380
heparin plasma(n=5)78-9586

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的血管紧张素转化酶2(ACE2)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管紧张素转化酶2(ACE2)含量的测定值与理论值的比率。

样本1:21:41:81:16
serum(n=5)84-92%83-97%80-95%97-104%
EDTA plasma(n=5)80-102%91-101%78-104%83-105%
heparin plasma(n=5)88-104%85-92%98-105%78-101%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将血管紧张素转化酶2(ACE2)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管紧张素转化酶2(ACE2)与连接于固相载体上的抗体结合，然后加入生物素化的血管紧张素转化酶2(ACE2)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管紧张素转化酶2(ACE2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号适用物种：Rattus norvegicus (Rat，大鼠)应用(仅供研究使用，不用于临床诊断！)
EPB886Ra61血管紧张素转化酶2(ACE2)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APB886Ra61血管紧张素转化酶2(ACE2)活性蛋白Cell culture; Activity Assays.
APB886Ra01血管紧张素转化酶2(ACE2)活性蛋白Cell culture; Activity Assays.
RPB886Ra01血管紧张素转化酶2(ACE2)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB886Ra02血管紧张素转化酶2(ACE2)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB886Ra01血管紧张素转化酶2(ACE2)多克隆抗体WB; IHC; ICC; IP.
MAB886Ra23血管紧张素转化酶2(ACE2)单克隆抗体WB; IHC; ICC; IP.
MAB886Ra22血管紧张素转化酶2(ACE2)单克隆抗体WB; IHC; ICC; IP.
MAB886Ra24血管紧张素转化酶2(ACE2)单克隆抗体WB; IHC; ICC; IP.
MAB886Ra25血管紧张素转化酶2(ACE2)单克隆抗体ICC/IF
MAB886Ra21血管紧张素转化酶2(ACE2)单克隆抗体WB; IHC; ICC; IP.
SEB886Ra血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB886Ra血管紧张素转化酶2(ACE2)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

参考文献

杂志参考文献
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Journal of Cancer TherapyPlasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression.[Scirp: Source]
Reprod Toxicol.?ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats[Pubmed:25205467]
Intensive Care Medicine Experimental Angiotensin converting enzymes in patients with acute respiratory distress syndrome[Content: 3]
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Innate Immunity Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis[Pubmed:29969931]
Journal of Surgical ResearchImpact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy[Pubmed:29506841]
canadian journal of physiology and pharmacologyCould cardioprotective effect of ACE2 activator “diminazene aceturate” is more potent than ACE inhibitor “Enalapril” on acute myocardial infarction in rats?[Pubmed: 30840489]
Intensive Care MedicineAngiotensin-converting enzymes in acute respiratory distress syndrome[]
BioMed Research InternationalAVE 0991 Attenuates Pyroptosis and Liver Damage after Heatstroke by Inhibiting the ROS-NLRP3 Inflammatory Signalling Pathway[]
Heart and vesselsRelationship between circulating levels of angiotensin-converting enzyme 2-angiotensin-(1–7)-MAS axis and coronary heart disease[Pubmed: 31359146]
MedicineAlteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study[Pubmed: 32756181]
American Journal of Respiratory and Critical Care MedicineEquilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition[Pubmed: 32628511]
Clinical Research in CardiologyDeterminants of soluble angiotensin-converting enzyme 2 concentrations in adult patients with complex congenital heart disease[33280062]
Clinical & Translational ImmunologyPattern of circulating SARS©\CoV©\2©\specific antibody©\secreting and memory B©\cell generation in patients with acute COVID©\19[33552508]
researchsquareNovel transgenic mice with Cre-dependent co-expression of GFP and human ACE2: a safe tool for study of COVID-19 pathogenesis[33855640]
VirusesDifferent Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera[]
Sci RepImmunoreactivity of the SARS-CoV-2 entry proteins ACE-2 and TMPRSS-2 in murine models of hormonal manipulation, ageing, and cardiac injury[34907257]
European Journal of Clinical InvestigationAngiotensin‐converting enzyme 2 and Transmembrane protease serine 2 in female and male patients with end‐stage kidney disease[Pubmed:35366343]
MetabolitesRamipril Reduces Acylcarnitines and Distinctly Increases Angiotensin-Converting Enzyme 2 Expression in Lungs of Rats[Pubmed:35448480]