趋化素(CHEM)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Chemerin (CHEM)
TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2
- 编号SEA945Ra
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1,000pg/mL
- 灵敏度最小可检测剂量小于等于6.9pg/mL.
- 样本类型serum, plasma, tissue homogenates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 2873¥ 4104¥ 18468¥ 34884¥ 287280
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特异性
本试剂盒用于检测趋化素(CHEM)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的趋化素(CHEM)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 85-92 | 89 |
| EDTA plasma(n=5) | 91-99 | 94 |
| heparin plasma(n=5) | 86-101 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的趋化素(CHEM)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中趋化素(CHEM)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-104% | 97-105% | 80-102% | 83-95% |
| EDTA plasma(n=5) | 88-95% | 81-98% | 88-101% | 87-95% |
| heparin plasma(n=5) | 83-103% | 93-101% | 96-104% | 85-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将趋化素(CHEM)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的趋化素(CHEM)与连接于固相载体上的抗体结合，然后加入生物素化的趋化素(CHEM)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的趋化素(CHEM)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA945Ra01 | 趋化素(CHEM)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA945Ra01 | 趋化素(CHEM)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA945Ra91 | 趋化素(CHEM)多克隆抗体(辣根过氧化物酶标记) | WB; IHC; ICC. |
| MAA945Ra24 | 趋化素(CHEM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA945Ra23 | 趋化素(CHEM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA945Ra22 | 趋化素(CHEM)单克隆抗体 | WB |
| MAA945Ra25 | 趋化素(CHEM)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA945Ra21 | 趋化素(CHEM)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA945Ra | 趋化素(CHEM)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA945Ra | 趋化素(CHEM)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Scandinavian Journal of Gastroenterology | Serum chemerin and vaspin in non-alcoholic fatty liver disease[PubMed: 20095887] |
| Gastroenterology & Hepatology | Chemerin, vaspin and insulin resistance in chronic hepatitis C[Wiley: source] |
| Placenta | Expression and regulation of chemerin during rat pregnancy[ScienceDirect: S0143400412000902] |
| Acta Pharmacologica Sinica | Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and rats[Nature: 201211] |
| RAED dergisi | Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnci[Raeddergisi: Source] |
| Journal of Cellular and Molecular Medicine | Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patients[Pubmed: 24702860] |
| Clinical Endocrinology | Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects[Pubmed: 24303851] |
| Oral Dis. | Impact of OSA on Biological Markers in Morbid Obesity and Metabolic Syndrome[Pubmed:24106991] |
| Endocrine | Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia[Pubmed:24840719] |
| The Clinical Respiratory Journal | Relationship between plasma chemerin levels and disease severity in COPD patients[Pubmed:24865134] |
| Gynecol Endocrinol. | Elevated serum chemerin in Chinese women with hyperandrogenic PCOS[Pubmed:24927079] |
| Endocrine. | Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats.[Pubmed:25471281] |
| Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats[Pubmed:25697126] |
| Dermatologica Sinica | Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgaris[Sciencedirect:S1027811714000470] |
| Dis Markers | Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control Study[PubMed: 26170530] |
| Med Sci Monit | Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese Population[PubMed: 26471865] |
| Endocrine | Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats[PubMed: 25471281] |
| Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes[Pubmed:26721419] |
| Medicine (Baltimore). | Association of chemerin levels and bone mineral density in Chinese obese postmenopausalwomen.[pubmed:27583869] |
| Oxidative Medicine and Cellular Longevity | Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …[Pubmed:29849871] |
| Current Eye Research | Chemerin is associated with diabetic retinopathy in type 2 diabetes[Pubmed:25848840] |
| Journal of Oral Rehabilitation | Expression of chemerin in the synovial fluid of patients with temporomandibular joint disorders[Pubmed:29331039] |
| International Journal of Cancer | Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancer[Pubmed: 30252132] |
| Experimental Gerontology | The decline of whole-body glucose metabolism in ovariectomized rats[Pubmed: 30292771] |
| Pancreatology | Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-κB signal[Doi: 10.1016/j.pan.2019.02.005] |
| Hypertens Pregnancy | Protective effect of vitamin D supplementation in a rat modal of preeclampsia: a possible implication of chemerin[Pubmed: 30922121] |
| Pancreatology | Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-kB signal[Pubmed: 30833212] |
| Digestion | Association between High Levels of Circulating Chemerin and Colorectal Adenoma in Men[Pubmed: 31269491] |
| Vitamin D Supplementation Reduces Serum Chemerin Level in Gestational Diabetes Mellitus Rat Model[] | |
| Aging Medicine | Plasma levels of adiponectin and chemerin are associated with early stage of atherosclerosis in older adults with type 2 diabetes mellitus[] |
| Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren[33575861] |
| Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren[33834274] |
| Indian Journal of Forensic Medicine & Toxicology | Altered Serum Marker of Adipokines Profile in Breast Cancer Women[] |
| Int J Womens Health | Association of High Serum Chemerin with Bone Mineral Density Loss and Osteoporotic Fracture in Elderly Chinese Women[Pubmed:35140527] |
