基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Matrix Metalloproteinase 13 (MMP13)
CLG3; Collagenase 3
产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测基质金属蛋白酶13(MMP13)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的基质金属蛋白酶13(MMP13)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 80-98 | 86 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的基质金属蛋白酶13(MMP13)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中基质金属蛋白酶13(MMP13)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-104% | 93-101% | 86-94% | 85-96% |
| heparin plasma(n=5) | 78-92% | 80-93% | 94-103% | 91-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将基质金属蛋白酶13(MMP13)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的基质金属蛋白酶13(MMP13)与连接于固相载体上的抗体结合，然后加入生物素化的基质金属蛋白酶13(MMP13)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的基质金属蛋白酶13(MMP13)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| EPA099Ra61 | 基质金属蛋白酶13(MMP13)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA099Ra01 | 基质金属蛋白酶13(MMP13)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA099Ra02 | 基质金属蛋白酶13(MMP13)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA099Ra61 | 基质金属蛋白酶13(MMP13)活性蛋白 | Cell culture; Activity Assays. |
| PAA099Ra01 | 基质金属蛋白酶13(MMP13)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA099Ra02 | 基质金属蛋白酶13(MMP13)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA099Ra71 | 基质金属蛋白酶13(MMP13)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA099Ra | 基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA099Ra | 基质金属蛋白酶13(MMP13)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA099Ra | 基质金属蛋白酶13(MMP13)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression[ScienceDirect: S0925443911001323] |
| Journal of Physiology and Biochemistry | Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats[SpringerLink: h875w73171w2147w] |
| FEBS Journal | Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta[Wiley: source] |
| Arthritis & Rheumatism | Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice[Wiley: source] |
| Gene | Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors[ScienceDirect: S0378111911007347] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| Arthritis Rheumatol | Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression.[Pubmed: 24578232] |
| Arthritis & Rheumatology | Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression[Onlinelibrary: art.38576] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke[Pubmed:24671655] |
| Arthritis & Rheumatol | Identification of α2-Macroglobulin as a Master Inhibitor of Cartilage-Degrading Factors That Attenuates the Progression of Posttraumatic Osteoarthritis[Pubmed:24578232] |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis[Pubmed:25448261] |
| Cellular Physiology and Biochemistry | Inverse Regulation of Early and Late Chondrogenic Differentiation by Oxygen Tension Provides Cues for Stem Cell-Based Cartilage Tissue Engineering[Pubmed:25632940] |
| Osteoarthritis and Cartilage | Depletion of gangliosides enhances cartilage degradation in mice[Sciencedirect:S1063458413010364] |
| Biol Res | The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis[PubMed: 26578310] |
| PLoS One | Interleukin-1 Receptor Antagonist Has a Novel Function in the Regulation of Matrix Metalloproteinase-13 Expression[PubMed: 26474296] |
| Cell Mol Immunol | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica[Pubmed:27157496] |
| Scientific Reports | Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds[pubmed:27821864] |
| International Journal of Contemporary Medical Research | Matrix Metalloproteinase-13 (MMP-13): A Novel Tumor Marker for Diagnosis of Breast Carcinoma[uploads:7] |
| Scientific Reports | Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption.[pubmed:27640363] |
| Photodermatol Photoimmunol Photomed. | Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice[pubmed:27995657] |
| Molecular Medicine Reports | Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells[pubmed:28447732] |
| bmc musculoskeletal disorders | Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis.[pubmed:28187731] |
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models[pubmed:27739057] |
| BMC Musculoskeletal Disorders | Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture[pubmed:29149841] |
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208] |
| Alexandria Journal of Medicine | L-Carnitine ameliorates knee lesions in mono-iodoacetate induced osteoarthritis in rats[10.1016:j.ajme.2016.03.002] |
| National Journal of Integrated Research in Medicine | Matrix Metalloproteinase-13 Can Be Used As an Independent Diagnostic and Prognostic Marker for Breast Carcinoma.[AN=123116960] |
| Molecular Medicine Reports | Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits[Pubmed:29257344] |
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis[10.1080:09712119.2017.1335208] |
| Osteoarthritis Cartilage | Coordinated existence of multiple gangliosides is required for cartilage metabolism[Pubmed: 30471358] |
| PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis[Pubmed: 30939166] |
| Pathobiology | Suppressed Expression but Not Activity of Collagenases MMP-1 and MMP-13 in Human Renal Carcinoma[Pubmed: 31129674] |
| Bone | DAla2GIP antagonizes H2O2-induced chondrocyte apoptosis and inflammatory factor secretion[Pubmed: 31283994] |
| Food & Function | A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese …[] |
| JOURNAL OF CELLULAR BIOCHEMISTRY | Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes[Pubmed: 32162384] |
| Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis[Pubmed: 32047705] |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis[Pubmed: 31980722] |
| Nutrients | Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial[Pubmed: 31888255] |
| Nature Communications | Vulnerability of progeroid smooth muscle cells to biomechanical forces is mediated by MMP13[Pubmed: 32807790] |
| Molecular Medicine Reports | MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression[Pubmed: 32705199] |
| RESEARCH SQUARE | Experimental Observation of the Sequence of Tibial Plateau Chondrocyte and Matrix Degeneration in Spontaneous Osteoarthritis in Guinea Pigs[] |
| Hyaluronic Acid versus Alpha-2-Macroglobulin Intra-Articular Injections for Amelioration of Knee Posttraumatic Osteoarthritis: A Rat Model[] | |
| Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs[] | |
| Food Funct | Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice[33890588] |
| Nutrients | Metabolic and Anti-Inflammatory Protective Properties of Human Enriched Serum Following Artichoke Leaf Extract Absorption: Results from an Innovative Ex Vivo …[34444810] |
| Sci Rep | Liraglutide, a glucagon-like peptide 1 receptor agonist, exerts analgesic, anti-inflammatory and anti-degradative actions in osteoarthritis[Pubmed:35091584] |
