S100钙结合蛋白B(S100B)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for S100 Calcium Binding Protein B (S100B)
S100-B; NEF; S100Beta; S-100 protein subunit beta
- 编号SEA567Rb
- 物种Oryctolagus cuniculus (Rabbit，兔)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1,000pg/mL
- 灵敏度最小可检测剂量小于等于6.3pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 2873¥ 4104¥ 18468¥ 34884¥ 287280
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产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测S100钙结合蛋白B(S100B)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的S100钙结合蛋白B(S100B)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 86-98 | 94 |
| heparin plasma(n=5) | 89-98 | 92 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的S100钙结合蛋白B(S100B)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中S100钙结合蛋白B(S100B)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-97% | 92-99% | 87-96% | 96-105% |
| EDTA plasma(n=5) | 93-105% | 94-103% | 78-104% | 86-97% |
| heparin plasma(n=5) | 88-104% | 96-103% | 78-99% | 79-89% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将S100钙结合蛋白B(S100B)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的S100钙结合蛋白B(S100B)与连接于固相载体上的抗体结合，然后加入生物素化的S100钙结合蛋白B(S100B)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的S100钙结合蛋白B(S100B)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Oryctolagus cuniculus (Rabbit，兔) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA567Rb01 | S100钙结合蛋白B(S100B)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA567Rb01 | S100钙结合蛋白B(S100B)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA567Rb52 | S100钙结合蛋白B(S100B)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA567Rb51 | S100钙结合蛋白B(S100B)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA567Rb71 | S100钙结合蛋白B(S100B)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA567Rb81 | S100钙结合蛋白B(S100B)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| SEA567Rb | S100钙结合蛋白B(S100B)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA567Rb | S100钙结合蛋白B(S100B)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA567Rb01 | S100钙结合蛋白B(S100B)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Epilepsia | Protective effects of naloxone in two-hit seizure model[PubMed: 19674055] |
| Journal of Neurotrauma | The Anti-Inflammatory and Neuroprotective Effects of Ghrelin in Subarachnoid Hemorrhage-Induced Oxidative Brain Damage in Rats[Liebert: 20091210] |
| Food and Chemical Toxicology | Sub-acute effects of diazinon on biochemical indices and specific biomarkers in rats: Protective effects of crocin and safranal[PubMed: 20637253] |
| 20 | Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder[Wiley: source] |
| Neuroscience Letters | A preliminary study of the effects of ulinastatin on early postoperative cognition function in patients undergoing abdominal surgery[Pubmed: 23422155] |
| Genes Cells | Induction of receptor for advanced glycation end products by insufficient leptin action triggers pancreatic β-cell failure in type 2 diabetes.[Pubmed: 23410183] |
| J Craniofac Surg. | The Experiment Research of Neuroprotection of Hypoperfusion Postconditioning on Cerebral Ischemia[Pubmed:25006960] |
| Int J Mol Sci | The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions[PubMed: 26184156] |
| Mol Neurobiol | The Severity of Cecal Ligature and Puncture-Induced Sepsis Correlates with the Degree of Encephalopathy, but the Sepsis Does Not Lead to Acute Activation of Spleen Lymphocytes in Mice[PubMed: 26081155] |
| Pediatria i Medycyna Rodzinna | Stężenie białka S100B w surowicy krwi u młodzieży z omdleniami[yadda:60e5630d0ac1] |
| 中国临床药理学与治疗学 | 右美托咪定对脑膜瘤切除术患者血清S100β 蛋白及神经元特异性烯醇化酶的影响[article:9794] |
| Eur J Pharmacol. | Development of an experimental rat model of hyperammonemic encephalopathy and evaluation of the effects of rifaximin[Pubmed:26980242] |
| Journal For Neurovirology | Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions.[pubmed:27882497] |
| Neurourol Urodyn | Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid[pubmed:27490041] |
| cellular physiology and biochemistry | Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β …[pubmed:29040978] |
| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms[pubmed:28061403] |
| Cellular Physiology and Biochemistry | Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory …[Pubmed:29672286] |
| Brain Research | Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathway[Pubmed:29510144] |
| Neurochemical Research | Protective Effects of Cornel Iridoid Glycoside in Rats After Traumatic Brain Injury[Pubmed:29492766] |
| Biochemical and Biophysical Research Communications | C6 glioma-conditioned medium induces malignant transformation of mesenchymal stem cells: Possible role of S100B/RAGE pathway[Pubmed:29050939] |
| Journal of the Neurological Sciences | S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid …[Pubmed:29801873] |
| Biochemistry and Cell Biology | Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsy[Pubmed:29365285] |
| S100B as a potential biomarker for concussion in male and female collegiate rugby athletes[:] | |
| Indian Journal of Psychiatry | Serum S100B levels in patients with depression[] |
| Evidence-Based Complementary and Alternative Medicine | Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway[] |
| Brain Sciences | Extracellular S100β Disrupts Bergman Glia Morphology and Synaptic Transmission in Cerebellar Purkinje Cells[Pubmed: 31013844] |
| Medicina | The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the …[] |
| ACTA NEUROBIOLOGIAE EXPERIMENTALIS | Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion …[Pubmed: 32214270] |
| Stem Cells International | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 …[Pubmed: 32300366] |
| Neurocritical Care | Biomarkers of Neurological Outcome After Aneurysmal Subarachnoid Hemorrhage as Early Predictors at Discharge from an Intensive Care Unit[Pubmed: 32978732] |
| European Review for Medical and Pharmacological Sciences | Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and …[Pubmed: 32432773] |
| ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ …[] | |
| Scientific Reports | Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD)[33469081] |
| Crit Care | Serum biomarkers and cerebral autoregulation as early warnings of delayed cerebral ischemia risk in patients after aneurysmal subarachnoid haemorrhage[27885969] |
| Transl Psychiatry | Increased GFAP concentrations in the cerebrospinal fluid of patients with unipolar depression[34021122] |
| Biochimie | Neuroprotective effect of 6-hydroxy-2, 2, 4-trimethyl-1, 2-dihydroquinoline mediated via regulation of antioxidant system and inhibition of inflammation and apoptosis in?¡[33964368] |
| Int J Neuropsychopharmacol | High S100B levels predict antidepressant response in patients with major depression even when considering inflammatory and metabolic markers[Pubmed:35176771] |
| Metab Brain Dis | 1-benzoyl-6-hydroxy-2, 2, 4-trimethyl-1, 2-dihydroquinoline exerts a neuroprotective effect and normalises redox homeostasis in a rat model of cerebral ischemia …[Pubmed:35201554] |
| PLoS One | Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats[Pubmed:35421180] |
| International Journal of Molecular Sciences | Sepsis Encephalopathy Is Partly Mediated by miR370-3p-Induced Mitochondrial Injury but Attenuated by BAM15 in Cecal Ligation and Puncture Sepsis Male Mice[Pubmed:35628259] |
| Authorea | THE EVALUATION OF MEDULLA SPINALIS PERFUSION AT MODERATE HYPOTHERMIA WITH PATIENTS WHO UNDERGO ANTEGRADE SELECTIVE CEREBRAL …[] |
| EPIDEMIOLOGIA, DETERMINANTELE ȘI PREDICȚIA ACCIDENTULUI VASCULAR CEREBRAL ISCHEMIC LA COPII[] |
