武汉云克隆科技股份有限公司

血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Von Willebrand Factor (vWF)

F8VWF; VWD; von Willebrand antigen 2

  • 血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法)实验结果图
  • SEA833Ra.jpg标准曲线图
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管性血友病因子(vWF)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管性血友病因子(vWF)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
sodium citrate plasma(n=5)82-9890

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的血管性血友病因子(vWF)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管性血友病因子(vWF)含量的测定值与理论值的比率。

样本1:21:41:81:16
sodium citrate plasma(n=5)82-96%92-101%83-99%78-99%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将血管性血友病因子(vWF)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管性血友病因子(vWF)与连接于固相载体上的抗体结合，然后加入生物素化的血管性血友病因子(vWF)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管性血友病因子(vWF)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号适用物种：Rattus norvegicus (Rat，大鼠)应用(仅供研究使用，不用于临床诊断！)
RPA833Ra02血管性血友病因子(vWF)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA833Ra01血管性血友病因子(vWF)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
CPA833Ra21血管性血友病因子(vWF)卵白蛋白偶联物Immunogen; SDS-PAGE; WB.
PAA833Ra01血管性血友病因子(vWF)多克隆抗体WB; IHC; ICC/IF
PAA833Ra02血管性血友病因子(vWF)多克隆抗体WB; IHC
PAA833Ra08血管性血友病因子(vWF)多克隆抗体WB; IHC; ICC; IP.
LAA833Ra71血管性血友病因子(vWF)多克隆抗体(生物素标记)WB; IHC; ICC.
LAA833Ra81血管性血友病因子(vWF)多克隆抗体(异硫氰酸荧光素标记)WB; IHC; ICC; IF.
MAA833Ra21血管性血友病因子(vWF)单克隆抗体WB
SEA833Ra血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
CEA833Ra血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA833Ra血管性血友病因子(vWF)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSA833Ra11血管性血友病因子(vWF)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

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