雌激素受体α(ERa)重组蛋白
Recombinant Estrogen Receptor Alpha (ERa)
ER-A1; ER; ESR; ESR1; ESRA; NR3A1; NR3-A1; Estrogen Receptor 1; Nuclear Receptor Subfamily 3,Group A,Member 1
- 编号RPB050Ra01
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位细胞膜, 细胞核, 细胞质, 染色体
- 预测分子量57.0kDa
- 实际分子量57kDa(差异分析请参阅说明书)
- 片段与标签Arg248~Leu484 with N-terminal His and GST Tag
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 97%
- 等电点7.1
- 应用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，请参见活性蛋白。 - 下载英文说明书 中文说明书
- 规格10µg50µg200µg1mg5mg
- 价格¥ 1152¥ 2880¥ 5760¥ 17280¥ 43200
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
Figure . Gene Sequencing (extract)
SDS-PAGE图
通过ISO 9001、ISO 13485质量体系认证
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB050Ra01 | 雌激素受体α(ERa)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB050Ra01 | 雌激素受体α(ERa)多克隆抗体 | WB; IHC; ICC; IP. |
| MAB050Ra21 | 雌激素受体α(ERa)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB050Ra | 雌激素受体α(ERa)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB050Ra | 雌激素受体α(ERa)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| The Journal of Steroid Biochemistry and Molecular Biology | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling[Pubmed:24846829] |
| Biochem Biophys Res Commun | Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomas[PubMed: 26529545] |
| Journal of Environmental Sciences | Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts[science:S1001074216300924] |
| cellular microbiology | Influenza A virus NS1 protein-induced JNK activation and apoptosis are not functionally linked.[pubmed:28076660] |
| Journal of Steroid Biochemistry and Molecular Biology | Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling[pubmed:28263910] |
| Cellular Physiology and Biochemistry | Antiestrogenic Activity of the Xi-Huang Formula for Breast Cancer by Targeting the Estrogen Receptor α[Pubmed:29975948] |
| Carleton University Research Virtual Environment | In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts[] |
| Clinical Breast Cancer | Vitamin D receptor in breast cancer tissues and its relation to estrogen receptor alpha (ER-a) gene expression and serum 25-hydroxyvitamin D levels in Egyptian [Doi: 10.1016/j.clbc.2018.12.019] |
| Journal of geriatric psychiatry and neurology | ERα Gene Promoter Methylation in Cognitive Function and Quality of Life of Patients With Alzheimer Disease[Pubmed: 30947592] |
| mBio | The Effector Domain of the Influenza A Virus Nonstructural Protein NS1 Triggers Host Shutoff by Mediating Inhibition and Global Deregulation of Host Transcription …[34488451] |
| Cardiometry | Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue[] |