催产素(OT)检测试剂盒(酶联免疫吸附试验法,高敏型)
High Sensitive ELISA Kit for Oxytocin (OT)
Pitocin; Syntocinon
特异性
本试剂盒用于检测催产素(OT)检测试剂盒(酶联免疫吸附试验法,高敏型)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的催产素(OT)检测试剂盒(酶联免疫吸附试验法,高敏型)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 78-98 | 87 |
| EDTA plasma(n=5) | 92-101 | 96 |
| heparin plasma(n=5) | 91-98 | 94 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的催产素(OT)检测试剂盒(酶联免疫吸附试验法,高敏型)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中催产素(OT)检测试剂盒(酶联免疫吸附试验法,高敏型)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-103% | 86-103% | 82-95% | 93-102% |
| EDTA plasma(n=5) | 85-97% | 87-94% | 88-105% | 95-102% |
| heparin plasma(n=5) | 84-98% | 96-105% | 80-92% | 84-99% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将催产素(OT)检测试剂盒(酶联免疫吸附试验法,高敏型)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPB052Ge11 | 催产素(OT)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPB052Ge21 | 催产素(OT)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAB052Ge01 | 催产素(OT)多克隆抗体 | ELISA\ |
| MEB052Ge | 催产素(OT)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEB052Ge | 催产素(OT)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEB052Ge | 催产素(OT)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB052Ge | 催产素(OT)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB052Ge11 | 催产素(OT)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| J Psychiatr Res.? | Sex-specific associations between plasma oxytocin levels and schizotypal personality features in healthy individuals[Pubmed:24411593] |
| Fertility and Sterility | Administration of atosiban in patients with endometriosis undergoing frozenâthawed embryo transfer: a prospective, randomized study[Pubmed:27143518] |
| Progress in neuro-psychopharmacology & biological psychiatry | Increased oxytocin levels among abstinent heroin addicts: Association with aggressiveness, psychiatric symptoms and perceived childhood neglect.[pubmed:28093220] |
| International Journal of Clinical and Experimental Medicine | Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic …[files:ijcem0031358.pdf] |
| Fertility and Sterility | Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study[pubmed:27143518] |
| BMC Med. | Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial.[pubmed:28209155] |
| Psychiatry Research | Plasma oxytocin levels in major depressive and bipolar II disorders[pubmed:28865715] |
| BMC Medicine | Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial[10.1186:s12916-017-0801-0] |
| EUROPEAN ADDICTION RESEARCH | The Association between the Level of Plasma Oxytocin and Craving among Former Heroin Users[Pubmed:29902803] |
| ASN Neuro . | Involvement of Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 in Oxytocin Neuronal Activity in Lactating Rats With Pup Deprivation[Pubmed: 32962418] |
| European Journal of Integrative Medicine | Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized …[] |
| J. Pers. Med | Predicting the Treatment Outcomes of Antidepressants Using a Deep Neural Network of Deep Learning in Drug-Naïve Major Depressive Patients[] |
