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多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)

High Sensitive ELISA Kit for Syndecan 1 (SDC1)

CD138; SDC; SYND1; Syndecan Proteoglycan 1

  • 多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)产品包装(模拟)
  • 多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)产品包装(模拟)
  • 多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)实验结果图
  • HEB966Hu.jpg标准曲线图
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
serum(n=5)84-9891
EDTA plasma(n=5)89-10194
heparin plasma(n=5)83-9287

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)含量的测定值与理论值的比率。

样本1:21:41:81:16
serum(n=5)80-99%79-94%79-99%93-102%
EDTA plasma(n=5)87-101%95-103%78-101%96-103%
heparin plasma(n=5)94-105%80-96%89-98%87-95%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)与连接于固相载体上的抗体结合，然后加入生物素化的多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法,高敏型)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
EPB966Hu62多配体蛋白聚糖1(SDC1)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
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MAB966Hu21多配体蛋白聚糖1(SDC1)单克隆抗体WB; IHC
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MAB966Hu24多配体蛋白聚糖1(SDC1)单克隆抗体WB; IHC; ICC; IP.
MAB966Hu25多配体蛋白聚糖1(SDC1)单克隆抗体WB
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MAB966Hu27多配体蛋白聚糖1(SDC1)单克隆抗体WB; IHC; ICC; IP.
MAB966Hu29多配体蛋白聚糖1(SDC1)单克隆抗体WB; IHC; ICC; IP.
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KSB966Hu01多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
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Mediators InflammDexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding[PubMed: 26199464]
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Arteriosclerosis, Thrombosis, and Vascular BiologyMPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic charge[Pubmed:29903730]
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Hepatobiliary & Pancreatic Diseases InternationalIncreased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICP[Pubmed: 31919038]
Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study[]
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Exp AnimDexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock model[Pubmed:35110424]
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