胰岛素降解酶(IDE)重组蛋白
Recombinant Insulin Degrading Enzyme (IDE)
Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase
- 编号RPB897Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位细胞核, 分泌, 细胞质
- 预测分子量29.7kDa
- 实际分子量30kDa(差异分析请参阅说明书)
- 片段与标签Ala753~Pro973 with N-terminal His Tag
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点7.2
- 应用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，请参见活性蛋白。 - 下载英文说明书 中文说明书
- 规格10µg50µg200µg1mg5mg
- 价格¥ 1166¥ 2916¥ 5832¥ 17496¥ 43740
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB897Hu01 | 胰岛素降解酶(IDE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB897Hu01 | 胰岛素降解酶(IDE)活性蛋白 | Cell culture; Activity Assays. |
| PAB897Hu01 | 胰岛素降解酶(IDE)多克隆抗体 | WB; IHC |
| MAB897Hu21 | 胰岛素降解酶(IDE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB897Hu22 | 胰岛素降解酶(IDE)单克隆抗体 | WB |
| SEB897Hu | 胰岛素降解酶(IDE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB897Hu | 胰岛素降解酶(IDE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB897Hu01 | 胰岛素降解酶(IDE)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| J Neurochem | Reduction[PubMed: 26442993] |
| African Journal of Pharmacy and Pharmacology | Peptide hydroxamate derivatives as regulators for insulin receptor signaling and its degradation by zinc metalloprotease in diabetic rats[article-full-text-pdf] |
| Biomedicine & Pharmacotherapy | Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats.[pubmed:27930980] |
| Biological Trace Element Research | Nigella sativa Oil and Chromium Picolinate Ameliorate Fructose-Induced Hyperinsulinemia by Enhancing Insulin Signaling and Suppressing Insulin-Degrading Enzyme in Male Rats[10.1007/s12011-017-1167-z] |
| Neuroscience | The Protective Effects of IGF-I against β-Amyloid-related Downregulation of Hippocampal Somatostatinergic System Involve Activation of Akt and Protein Kinase A[Pubmed:29406271] |