糖化血红蛋白A1c(HbA1c)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Glycated Hemoglobin A1c (HbA1c) ,etc. by FLIA (Flow Luminescence Immunoassay)
Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc
（注：单次混测多因子不超过8个指标 ）
- 编号LMA190Po
- 物种Sus scrofa; Porcine (Pig，猪)相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围0.9766-1000ug/mL
- 灵敏度最小可检测剂量小于等于0.3255ug/mL.
- 样本类型plasma and erythrocyte lysates
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特异性
本试剂盒用于检测糖化血红蛋白A1c(HbA1c)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的糖化血红蛋白A1c(HbA1c)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 95-103 | 101 |
| EDTA plasma(n=5) | 78-101 | 88 |
| heparin plasma(n=5) | 93-101 | 97 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的糖化血红蛋白A1c(HbA1c)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中糖化血红蛋白A1c(HbA1c)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-95% | 87-101% | 85-92% | 80-105% |
| EDTA plasma(n=5) | 80-104% | 88-102% | 85-95% | 80-90% |
| heparin plasma(n=5) | 93-101% | 84-96% | 92-105% | 78-97% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将糖化血红蛋白A1c(HbA1c)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的糖化血红蛋白A1c(HbA1c)等多因子检测试剂盒(流式荧光发光法)和未标记的糖化血红蛋白A1c(HbA1c)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的糖化血红蛋白A1c(HbA1c)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Sus scrofa; Porcine (Pig，猪) | 应用(仅供研究使用，不用于临床诊断！) |
| CEA190Po | 糖化血红蛋白A1c(HbA1c)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA190Po | 糖化血红蛋白A1c(HbA1c)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| J Am Coll Nutr | The Effect of Vitamin D Supplementation on Glycemic Control and Lipid Profile in Patients with Type 2 Diabetes Mellitus[PubMed: 26391639] |
| Journal of the American College of Nutrition | The Effect of Vitamin D Supplementation on Glycemic Control and Lipid Profile in Patients with Type 2 Diabetes Mellitus.[pubmed:26391639] |
| Journal of Endocrinology and Metabolism | Circulating Betatrophin and Hepatocyte Growth Factor in Type 2 Diabetic Patients: Their Relationship With Disease Prognosis[405] |
| Molecular nutrition & food research | Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro[pubmed:28105758] |
| 33 | d-Ribose as a Contributor to Glycated Haemoglobin[pubmed:29033370] |
| Journal of Functional Foods | Hypoglycemic effect of Hypericum attenuatum Choisy extracts on type 2 diabetes by regulating glucolipid metabolism and modulating gut microbiota[] |
| West Indian Medical Journal | Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.[] |
| Journal of Occupational and Environmental Hygiene | Stressors, allostatic load, and health outcomes among women hotel housekeepers: A pilot study[Pubmed: 30615593] |
| Cell Reports | Parabacteroides distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile Acids[Pubmed: 30605678] |
| Journal of Agricultural and Food Chemistry | Gymnemic acid alleviates type 2 diabetes mellitus and suppresses endoplasmic reticulum stress in vivo and in vitro[] |
| Journal of Clinical Medicine | 1H Nuclear Magnetic Resonance (NMR)-Based Cerebrospinal Fluid and Plasma Metabolomic Analysis in Type 2 Diabetic Patients and Risk Prediction for Diabetic …[Pubmed: 31248127] |
| Food & Function | Gymnemic Acid Alleviates Inflammation and Insulin Resistance via PPARδ-and NFκB-mediated pathways in db/db mice.[] |
| Obesity Surgery | Duodenal-Jejunal Bypass Ameliorates Type 2 Diabetes Mellitus by Activating Insulin Signaling and Improving Glucose Utilization in the Brain[Pubmed: 31605365] |
| Acta Diabetologica | Modulation of gut microbiota contributes to effects of intensive insulin therapy on intestinal morphological alteration in high-fat-diet-treated mice[Pubmed: 31749050] |
| Open Access Macedonian Journal of Medical Sciences | Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy[Pubmed: 32165929] |
| Cell Reports | Activation of a Specific Gut Bacteroides-Folate-Liver Axis Benefits for the Alleviation of Nonalcoholic Hepatic Steatosis[Pubmed: 32783933] |
| The effects of different intensity endurance and resistance exercise on diabetic-related blood profiles in impaired glucose tolerance mice[] | |
| Revista Internacional de Andrologia | Relation of pharmacopenile duplex ultrasonography parameters and glycated hemoglobin (HbA1c) in diabetic patients with erectile dysfunction[33334711] |
| Advanced Theory and Simulations? | Identification of Key Contributive Compounds in a Herbal Medicine: A Novel Mathematic¡ªBiological Evaluation Approach[] |
| Dis Markers | The Roles of Liver Inflammation and the Insulin Signaling Pathway in PM2. 5 Instillation-Induced Insulin Resistance in Wistar Rats[34745386] |
| Antioxidants | Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice[Pubmed:35740008] |
| P001–Inv. Clínica[] |