降钙素原(PCT)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Procalcitonin (PCT) ,etc. by FLIA (Flow Luminescence Immunoassay)
Pro-Calcitonin
（注：单次混测多因子不超过8个指标 ）
- 编号LMA689Rb
- 物种Oryctolagus cuniculus (Rabbit，兔)相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围1.9531-2000pg/mL
- 灵敏度最小可检测剂量小于等于0.651pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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特异性
本试剂盒用于检测降钙素原(PCT)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的降钙素原(PCT)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 99-105 | 102 |
| EDTA plasma(n=5) | 78-97 | 85 |
| heparin plasma(n=5) | 91-99 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的降钙素原(PCT)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中降钙素原(PCT)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-92% | 78-101% | 83-93% | 82-95% |
| EDTA plasma(n=5) | 81-93% | 95-103% | 96-104% | 87-102% |
| heparin plasma(n=5) | 86-93% | 82-98% | 78-102% | 81-92% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将降钙素原(PCT)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的降钙素原(PCT)等多因子检测试剂盒(流式荧光发光法)和未标记的降钙素原(PCT)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的降钙素原(PCT)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Oryctolagus cuniculus (Rabbit，兔) | 应用(仅供研究使用，不用于临床诊断！) |
| EPA689Rb61 | 降钙素原(PCT)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CEA689Rb | 降钙素原(PCT)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA689Rb | 降钙素原(PCT)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Scandinavian Journal of Infectious Diseases | Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis[PubMed: 20085423] |
| International Journal of Radiation Oncology ? Biology ? Physics | Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice[PubMed: 20637981] |
| Ulus Travma Acil Cerrahi Derg | Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia[PubMed: 21935794] |
| Egyptian Journal of Bronchology | Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children[Essbronchology: Source] |
| University of Waikato | The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis[Waikato:Source] |
| Egyptian Journal of Medical Microbiology | Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis[Ejmm:Source] |
| Nigerian Medical Journal | Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis[Pubmed:25657488] |
| Wiley online library | The use and value of procalcitonin in solid organ transplantation[PubMed: 25996831] |
| Balkan Med J | Diagnostic Value of Procalcitonin Levels in Acute Mesenteric Ischemia[PubMed: 26185718] |
| The Journal of Clinical Endocrinology & Metabolism | Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay[PubMed: 26647152] |
| Journal of Human Hypertension | Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension[PubMed: 26040439] |
| Medical Science Monitor | Variation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection.[Pubmed:26772168] |
| International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery[Pubmed:26802602] |
| Pancreatology. | Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis.[Pubmed:26804005] |
| Acta Medica | Risk Factors of Acute Pancreatitis in Oral Double Balloon Enteroscopy.[pubmed:27638962] |
| The Clinical Respiratory Journal | Changes in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndrome[pubmed:28960823] |
| SHOCK | INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATION[pubmed:27984536] |
| Cellular Physiology and Biochemistry | Hepatic Macrophages are the Cell Source of Hepatic Procalcitonin in Acute Liver Failure[Pubmed:29913443] |
| Archives of Medical Science | Inflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidase[Pubmed:29593801] |
| Journal of Thoracic Disease | Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung[Pubmed:29707313] |
| Asian Journal of Psychiatry | Procalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patients[Pubmed:29723719] |
| European Review for Medical and Pharmacological Sciences | The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia.[Pubmed: 31298341] |
| Medicina | Modestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active Infection[Pubmed: 33080909] |
| MEDIATORS OF INFLAMMATION | Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase[Pubmed: 32410851] |
| Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes[] | |
| Journal of Immunology Research | Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis[33628853] |
| Journal of Medical Biochemistry | Circulating nuclear factor-kappa B mediates cancer-associated inflammation in human breast and colon cancer[33776564] |
| Surg Endosc | Original contribution: sleeve gastrectomy reduces soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in patients with morbid obesity[Pubmed:35044516] |
| Bone Res | Procalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatment[Pubmed:35087025] |