武汉云克隆科技股份有限公司

血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Angiotensin 1-7 (Ang1-7) ,etc. by FLIA (Flow Luminescence Immunoassay)

（注：单次混测多因子不超过8个指标 ）

    • 血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)产品包装(模拟)
    • 血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)产品包装(模拟)
    • Certificate通过ISO 9001、ISO 13485质量体系认证

    特异性

    本试剂盒用于检测血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
    由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

    回收率

    分别于定值血清及血浆样本中加入一定量的血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

    样本回收率范围(%)平均回收率(%)
    serum(n=5)93-10298
    EDTA plasma(n=5)84-9993
    heparin plasma(n=5)92-10195

    精密度

    精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
    批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
    批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
    批内差： CV<10%
    批间差： CV<12%

    线性

    在定值血清及血浆样本内加入适量的血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

    样本1:21:41:81:16
    serum(n=5)86-94%93-101%84-101%80-89%
    EDTA plasma(n=5)78-99%88-99%88-102%93-102%
    heparin plasma(n=5)82-94%85-96%94-104%92-99%

    稳定性

    经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
    为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

    实验流程

    1. 实验前标准品、试剂及样本准备；
    2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
        37°C酶标板振荡器孵育60分钟；
    3. 磁吸洗板3次；
    4. 加检测溶液B100μL，37°C振动孵育30分钟；
    5. 磁吸洗板3次；
    6. 加鞘液100μL，旋涡震荡2分钟后读数。

    实验原理

    将血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)和未标记的血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)呈负相关。

    赠品

    相关产品

    编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
    CPS085Hu21血管紧张素1-7(Ang1-7)卵白蛋白偶联物Immunogen; SDS-PAGE; WB.
    PAS085Hu01血管紧张素1-7(Ang1-7)多克隆抗体ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
    LAS085Hu71血管紧张素1-7(Ang1-7)多克隆抗体(生物素标记)WB; IHC; ICC.
    CES085Hu血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
    CCS085Hu血管紧张素1-7(Ang1-7)检测试剂盒(化学发光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
    LMS085Hu血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
    KSS085Hu11血管紧张素1-7(Ang1-7)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

    参考文献

    杂志参考文献
    J Stroke Cerebrovasc DisImprovement of Plasma Biomarkers after Switching Stroke Patients from Other Angiotensin II Type I Receptor Blockers to Olmesartan[PubMed: 25891757]
    Cellular Physiology and BiochemistryAngiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats[pubmed:28848201]
    Frontiers in PhysiologyHydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression[pubmed:29066981]
    Molecular Medicine ReportsThe ACE2-Ang (1-7)-Mas receptor axis attenuates cardiac remodeling and fibrosis in post-myocardial infarction.[pubmed:28656296]
    Journal of Clinical Pharmacy and TherapeuticsRelationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites.[pubmed:28895159]
    Molecular Medicine ReportsEffects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats[pubmed:28944898]
    Open Journal of Endocrine and Metabolic Diseases Paper SubmissionA Study of the Correlation between Angiotensin (1-7) and the Histopathological Changes in the Penises of Experimentally Diabetic Rats[2-1980272_83198.htm]
    Journal of the American Society of HypertensionMesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system[Pubmed:29752040]
    Innate Immunity Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis[Pubmed:29969931]
    Evidence-based Complementary and Alternative Medicine Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects[Pubmed:29977313]
    European Journal of Obstetrics & Gynecology and Reproductive BiologyThe impact of ovulation inducing drugs on carcinogenesis[]
    European Journal of Obstetrics & Gynecology and Reproductive BiologyChanges of angiotensin II (1–8) and angiotensin (1–7) levels in early and late-onset preeclampsia[]
    Journals of Gerontology Series A-Biological Sciences and Medical SciencesTherapeutic Delivery of Ang (1-7) Via Genetically Modified Probiotic: A Dosing Study.[Pubmed: 31586210]
    journal of the european academy of dermatology and venereologySignificance of the angiotensin I/angiotensin II/angiotensin‐(1‐7) axis in the pathogenesis of systemic sclerosis[Pubmed: 31746507]
    MedicineAlteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study[Pubmed: 32756181]
    Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum …[]
    Amino AcidsAngiotensin-(1¨C7) attenuates collagen-induced arthritis via inhibiting oxidative stress in rats[33398523]
    AutoimmunityThe prevalence and role of functional autoantibodies to angiotensin-converting-enzyme-2 in patients with systemic sclerosis[33910447]
    researchsquareAng-(1-7) Protects Skeletal Muscle Function in Aged Mice[]
    PeptidesAngiotensin-(1-7) protects against sepsis-associated left ventricular dysfunction induced by lipopolysaccharide[34298021]
    BMC Musculoskeletal DisordersAng-(1–7) protects skeletal muscle function in aged mice[34548056]
    J Cardiovasc PharmacolResveratrol Confers Protection Against Ischemia/Reperfusion Injury by Increase of Angiotensin (1-7) Expression in a Rat Model of Myocardial Hypertrophy[34232225]