膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay)
ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV
（注：单次混测多因子不超过8个指标 ）
- 编号LMB944Mu
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.01-10ng/mL
- 灵敏度最小可检测剂量小于等于0.003 ng/mL.
- 样本类型Serum, plasma, tissue homogenates and other biological fluids
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 90-102 | 97 |
| EDTA plasma(n=5) | 90-97 | 94 |
| heparin plasma(n=5) | 86-96 | 92 |
| sodium citrate plasma(n=5) | 79-104 | 87 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-103% | 80-90% | 97-104% | 80-105% |
| EDTA plasma(n=5) | 82-102% | 81-104% | 84-96% | 93-101% |
| heparin plasma(n=5) | 85-99% | 90-97% | 93-101% | 85-101% |
| sodium citrate plasma(n=5) | 94-102% | 81-97% | 90-98% | 86-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB944Mu02 | 膜联蛋白A2(ANXA2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB944Mu01 | 膜联蛋白A2(ANXA2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB944Mu01 | 膜联蛋白A2(ANXA2)活性蛋白 | Cell culture; Activity Assays. |
| PAB944Mu01 | 膜联蛋白A2(ANXA2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB944Mu02 | 膜联蛋白A2(ANXA2)多克隆抗体 | WB; IHC; ICC; IP. |
| SEB944Mu | 膜联蛋白A2(ANXA2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB944Mu | 膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Cellular Biochemistry | Subcellular proteome analysis unraveled annexin A2 related to immune liver fibrosis[PubMed: 20225235] |
| World Journal of Gastroenterology | Expression characteristics and diagnostic value of annexin A2 in hepatocellular carcinoma[PubMed: PMC3491596] |
| Oncology Letters | Annexin A2 is not a good biomarker for hepatocellular carcinoma in cirrhosis[PubMed: PMC3742823] |
| Ibrahim | Annexin A2 Versus Afp as An Efficient Diagnostic Serum Marker for Hepatocellular Carcinoma[Ghrnet: Source] |
| European Journal of Clinical Microbiology & Infectious Diseases | Serum annexin A2 levels in acute brucellosis and brucellar spondylodiscitis[Pubmed:24853056] |
| Biomedicine & Pharmacotherapy | Circulating annexin A2 as a biomarker in gastric cancer patients: Correlation with clinical variables[Pubmed:25661364] |
| Hepat Mon | Serum Levels of Annexin A2 as a Candidate Biomarker for Hepatic Fibrosis in Patients With Chronic Hepatitis B[PubMed: 26587036] |
| Tumour Biol | Up-regulation of annexin A2 expression predicates advanced clinicopathological features and poor prognosis in hepatocellular carcinoma[PubMed: 26109000] |
| Biomed Pharmacother | Circulating[PubMed: 25661364] |
| Tumour Biol | Evaluation of annexin A2 and as potential biomarkers for hepatocellular carcinoma[PubMed: 26189841] |
| Eur J Clin Invest | Stairs instead of elevators at the workplace decreases PCSK9 levels in a healthy population[PubMed: 26081791] |
| Journal of Oral and Maxillofacial Surgery | Serum Annexin A2 Level Is Associated With Diagnosis and Prognosis in Patients With OralSquamous Cell Carcinoma.[pubmed:27889534] |
| Pregnancy Hypertens | Assessment of annexin A5 and annexin A2 levels as biomarkers for pre-eclampsia: A pilot study.[pubmed:28501283] |
| Mol Nutr Food Res. | Epigallocatechin gallate induces an up-regulation of LDL receptor accompanied by a reduction of PCSK9 via the annexin A2-independent pathway in HepG2 cells.[pubmed:28181408] |
| Archives of Gynecology and Obstetrics | Expression of annexin A2 in adenomyosis and dysmenorrhea[Pubmed: 31183557] |
| Cancers | Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer[Pubmed: 31842290] |
| PLASMA BIOMARKER FOR OVARIAN CANCER[] | |
| Diagnostics | Diagnostic Value of Plasma Annexin A2 in Early-Stage High-Grade Serous Ovarian Cancer. Diagnostics 2021, 11, 69[33406648] |
| ADVANCED RESEARCHES | Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C[] |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins[] |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression[34376212] |