骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Bone Morphogenetic Protein 2 (BMP2) ,etc. by FLIA (Flow Luminescence Immunoassay)
BMP2A; BMP-2A; Hemochromatosis Modifier
（注：单次混测多因子不超过8个指标 ）
- 编号LMA013Hu
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.98-1000pg/mL
- 灵敏度最小可检测剂量小于等于0.327 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 85-102 | 93 |
| EDTA plasma(n=5) | 84-99 | 88 |
| heparin plasma(n=5) | 97-105 | 101 |
| sodium citrate plasma(n=5) | 83-96 | 86 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-101% | 79-97% | 84-103% | 87-101% |
| EDTA plasma(n=5) | 92-101% | 94-105% | 97-104% | 79-98% |
| heparin plasma(n=5) | 97-104% | 85-101% | 79-94% | 86-104% |
| sodium citrate plasma(n=5) | 89-101% | 80-101% | 92-99% | 88-99% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA013Hu01 | 骨成型蛋白2(BMP2)活性蛋白 | Cell culture; Activity Assays. |
| RPA013Hu02 | 骨成型蛋白2(BMP2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA013Hu01 | 骨成型蛋白2(BMP2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA013Hu03 | 骨成型蛋白2(BMP2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA013Hu02 | 骨成型蛋白2(BMP2)活性蛋白 | Cell culture; Activity Assays. |
| PAA013Hu02 | 骨成型蛋白2(BMP2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA013Hu01 | 骨成型蛋白2(BMP2)多克隆抗体 | WB |
| MAA013Hu22 | 骨成型蛋白2(BMP2)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA013Hu | 骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA013Hu | 骨成型蛋白2(BMP2)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA013Hu | 骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA013Hu01 | 骨成型蛋白2(BMP2)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
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| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| Medical Journal of Chinese People's Liberation Army | Effect of transplantation of BMP-2-induced bone marrow mesenchymal stem cells on myocardial infarction of rats after reperfusion[Source] |
| PLOS ONE | PDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by down-regulating PDGFRα and derepressing BMP-Smad1/5/8 signaling[Pubmed:25470749] |
| Eur J Pharmacol | Negative effect of serotonin–norepinephrine reuptake inhibitor therapy on rat bone tissue after orchidectomy[PubMed: 25934570] |
| Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy[PubMed: 25871861] |
| Journal of Cellular Biochemistry | Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway[PubMed: 26681207] |
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| Research report | EFFECTS OF FLUORIDE ON THE EXPRESSION OF BMP-2 AND SMAD1 IN RAT OSTEOBLASTS IN VITRO[files:p013-022] |
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| Journal of Materials Chemistry B | Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage Repair[DOI:10.1039/C7TB00640C] |
| Scientific Reports | 3D- Printed Poly(ε-caprolactone) Scaffold Integrated with Cell-laden Chitosan Hydrogels for Bone Tissue Engineering.[pubmed:29042614] |
| Journal of Applied Biomaterials & Functional Materials | Demineralized dentin and enamel matrices as suitable substrates for bone regeneration[pubmed:28731486] |
| Journal of Clinical Periodontology | Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats[Pubmed:29574908] |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats[Pubmed:29898457] |
| Journal of Applied Biomaterials & Functional Materials | BMP-2 and type I collagen preservation in human deciduous teeth after demineralization[Pubmed:30045659] |
| Applied Surface Science | Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2 immobilized titanium surface through polymer-mediated electrostatic interaction[] |
| Materials Science and Engineering: C | Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration[] |
| Biomaterials Science | Development of CaCO 3 microsphere-based composite hydrogel for dual delivery of growth factor and Ca to enhance bone regeneration[] |
| ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats[Pubmed: 32004919] |
| Journal of Industrial and Engineering Chemistry | Biofabrication and application of decellularized bone extracellular matrix for effective bone regeneration[] |
| BMC Musculoskeletal Disorders | Synergistic Effect of Platelet-rich Fibrin Releasate and Bone Marrow Stem Cells in Preventing Bone Loss in Ovariectomized Mouse[] |
| Bioact Mater | New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density[33842739] |
| Tissue Eng Regen Med | Osteogenic and Angiogenic Potency of VEGF165-Transfected Canine Bone Marrow Mesenchymal Cells Combined with Coral Hydroxyapatite in Vitro[34302695] |
| Toxicon | The WNT/β-catenin signalling pathway induces chondrocyte apoptosis in the cartilage injury caused by T-2 toxin in rats[34742779] |
| ACS Biomater Sci Eng | Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum[34472855] |
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