18kDa阳离子抗菌肽(CAMP)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Cathelicidin Antimicrobial Peptide (CAMP) ,etc. by FLIA (Flow Luminescence Immunoassay)
CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic
（注：单次混测多因子不超过8个指标 ）
- 编号LMC419Mu
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.05-50ng/mL
- 灵敏度最小可检测剂量小于等于0.017 ng/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格8指标数7指标数6指标数5指标数4指标数3指标数2指标数1指标数
- 价格¥ 2696¥ 2799¥ 2955¥ 3162¥ 3370¥ 3681¥ 4147¥ 5184 添加到价格计算器 计算器
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测18kDa阳离子抗菌肽(CAMP)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的18kDa阳离子抗菌肽(CAMP)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 95-104 | 101 |
| EDTA plasma(n=5) | 91-98 | 94 |
| heparin plasma(n=5) | 87-94 | 91 |
| sodium citrate plasma(n=5) | 96-103 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的18kDa阳离子抗菌肽(CAMP)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中18kDa阳离子抗菌肽(CAMP)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-91% | 87-99% | 86-93% | 82-101% |
| EDTA plasma(n=5) | 90-99% | 85-92% | 80-92% | 90-99% |
| heparin plasma(n=5) | 82-101% | 80-92% | 79-89% | 80-88% |
| sodium citrate plasma(n=5) | 84-94% | 84-102% | 83-92% | 82-95% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将18kDa阳离子抗菌肽(CAMP)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的18kDa阳离子抗菌肽(CAMP)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的18kDa阳离子抗菌肽(CAMP)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的18kDa阳离子抗菌肽(CAMP)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| EPC419Mu61 | 18kDa阳离子抗菌肽(CAMP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC419Mu02 | 18kDa阳离子抗菌肽(CAMP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC419Mu01 | 18kDa阳离子抗菌肽(CAMP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC419Mu61 | 18kDa阳离子抗菌肽(CAMP)活性蛋白 | Cell culture; Activity Assays. |
| PAC419Mu02 | 18kDa阳离子抗菌肽(CAMP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAC419Mu01 | 18kDa阳离子抗菌肽(CAMP)多克隆抗体 | WB; IHC; ICC; IP. |
| SEC419Mu | 18kDa阳离子抗菌肽(CAMP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEC419Mu | 18kDa阳离子抗菌肽(CAMP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC419Mu | 18kDa阳离子抗菌肽(CAMP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSC419Mu01 | 18kDa阳离子抗菌肽(CAMP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
| KSC419Mu11 | 18kDa阳离子抗菌肽(CAMP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| University of Waikato | The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis[Waikato:Source] |
| Clin Infect Dis. | Host immune response to tuberculous meningitis[Pubmed:25301213] |
| Vaccine | On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment[Pubmed:25444792] |
| Urology | Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells [Pubmed:25681250] |
| Urology | Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells[PubMed: 25681250] |
| Sarcoidosis Vasc Diffuse Lung Dis | Cathelicidin as a link between sarcoidosis and tuberculosis[PubMed: 26422567] |
| Allergy and Asthma Proceedings | The association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in children[PubMed: 26108071] |
| J Clin Microbiol. | Swiftly Decreasing Cerebrospinal Fluid Cathelicidin Concentration Predicts Improved Outcome in Childhood Bacterial Meningitis.[Pubmed:27008883] |
| experimental dermatology | Light-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin.[pubmed:27315464] |
| PLOS ONE | Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension.[pubmed:27391973] |
| Front Pharmacol. | Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance.[pubmed:28261091] |
| Allergologia et immunopathologia | The relation of innate and adaptive immunity with viral-induced acute asthma attacks: Focusing on IP-10 and cathelicidin[pubmed:27955890] |
| Chinese Journal of Osteoporosis and Bone Mineral Research | Effects of cold exposure on the bone marrow adipose tissue[DOI: 10.3969/j.issn.1674-2591.2017.05.008] |
| Scientific Reports | Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique[Pubmed:29374283] |
| Experimental Parasitology | Antimalarial activity of vitamin D3 (VD3) does not result from VD3-induced antimicrobial agents including nitric oxide or cathelicidin[Pubmed: 30904694] |
| Theranostics | Myeloid cell-derived LL-37 promotes lung cancer growth by activating Wnt/β-catenin signaling[Pubmed: 31149039] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37[Pubmed: 32050475] |
| MICROB PATHOGENESIS | Hypovitaminosis D and reduced cathelicidin are strongly correlated during the multidrug therapy against leprosy[Pubmed: 32645421] |
| International Journal of Nanomedicine | Effect of Lonicerae japonicae Flos Carbonisata-Derived Carbon Dots on Rat Models of Fever and Hypothermia Induced by Lipopolysaccharide[Pubmed: 32606669] |
| 博士论文 | Relações causais entre a expressão dos genes do receptor de Vitamina D e do Peptídeo Antimicrobiano Catelicidina sobre marcadores sorológicos de pessoas com …[] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies[33999928] |
| New Med | Analysis of the level of non-specific and specific immunity parameters in saliva of children with osteogenesis imperfecta and study of relationships between?¡[] |
| Type I interferons link skin-associated dysbiotic commensal bacteria to pathogenic inflammation and angiogenesis in rosacea[] | |
| Int J Mol Sci | Compartmentalized Innate Immune Response of Human Fetal Membranes against Escherichia coli Choriodecidual Infection[Pubmed:35328414] |
| Cell Rep | LL-37 transports immunoreactive cGAMP to activate STING signaling and enhance interferon-mediated host antiviral immunity[Pubmed:35649354] |