Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ,etc. by FLIA (Flow Luminescence Immunoassay)
CTX-I; CTX1;
（注：单次混测多因子不超过8个指标 ）
- 编号LMA665Hu
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围9.77-10000pg/mL
- 灵敏度最小可检测剂量小于等于3.257 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格8指标数7指标数6指标数5指标数4指标数3指标数2指标数1指标数
- 价格¥ 2359¥ 2449¥ 2586¥ 2767¥ 2948¥ 3221¥ 3629¥ 4536 添加到价格计算器 计算器
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 90-102 | 99 |
| EDTA plasma(n=5) | 89-101 | 97 |
| heparin plasma(n=5) | 81-89 | 85 |
| sodium citrate plasma(n=5) | 90-104 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-94% | 91-98% | 96-104% | 91-98% |
| EDTA plasma(n=5) | 78-98% | 95-102% | 91-101% | 93-101% |
| heparin plasma(n=5) | 90-102% | 90-97% | 93-102% | 87-101% |
| sodium citrate plasma(n=5) | 80-102% | 98-105% | 78-98% | 83-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)和未标记的Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA665Hu21 | Ⅰ型胶原交联羧基端肽(CTXI)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA665Hu31 | Ⅰ型胶原交联羧基端肽(CTXI)钥孔血蓝蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| SPA665Hu01 | Ⅰ型胶原交联羧基端肽(CTXI)合成多肽 | Immunogen; Blocking Peptide. |
| CPA665Hu11 | Ⅰ型胶原交联羧基端肽(CTXI)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA665Hu01 | Ⅰ型胶原交联羧基端肽(CTXI)多克隆抗体 | WB; IHC |
| PAA665Hu02 | Ⅰ型胶原交联羧基端肽(CTXI)多克隆抗体 | WB; IHC |
| LAA665Hu71 | Ⅰ型胶原交联羧基端肽(CTXI)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA665Hu91 | Ⅰ型胶原交联羧基端肽(CTXI)多克隆抗体(辣根过氧化物酶标记) | WB; IHC; ICC. |
| LAA665Hu81 | Ⅰ型胶原交联羧基端肽(CTXI)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA665Hu21 | Ⅰ型胶原交联羧基端肽(CTXI)单克隆抗体 | WB; IHC |
| LAA665Hu84 | Ⅰ型胶原交联羧基端肽(CTXI)单克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| CEA665Hu | Ⅰ型胶原交联羧基端肽(CTXI)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA665Hu | Ⅰ型胶原交联羧基端肽(CTXI)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA665Hu11 | Ⅰ型胶原交联羧基端肽(CTXI)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA665Hu11 | Ⅰ型胶原交联羧基端肽(CTXI)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| journal of bone and mineral research | Atp6v0d2 Is an Essential Component of the Osteoclast-Specific Proton Pump That Mediates Extracellular Acidification in Bone Resorption[PubMed: PMC2672205] |
| Bone | Borage and fish oils lifelong supplementation decreases inflammation and improves bone health in a murine model of senile osteoporosis[PubMed: 21664309] |
| Trials | Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial.[Trials: 1745-6215] |
| Journal of Biological Chemistry | TRAF family member-associated NF-κB activator (TANK) is a negative regulator of osteoclastogenesis and bone formation[PubMed: 22773835] |
| The Journal of Clinical Endocrinology & Metabolism | A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular Risk[PubMed: PMC3462931] |
| Endocrinology | Oxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat Gain[Endocrine: Source] |
| Am J Physiol Endocrinol Metab. | DPP IV inhibitor treatment attenuates bone loss and improves mechanical bone strength in male diabetic rats[Pubmed:25053403] |
| The American Journal of Cardiology | Relation of burden of myocardial fibrosis to malignant ventricular arrhythmias and outcomes in Fabry disease[Pubmed:25073565] |
| Rheumatology (Oxford). | Bone resorption correlates with the frequency of CD5+ B cells in the blood of patients with rheumatoid arthritis[Pubmed:25193807] |
| XPO1/CRM1-Selective Inhibitors of Nuclear Export (SINE) reduce tumor spreading and improve overall survival in preclinical models of prostate cancer (PCa)[Pubmed:25284315] | |
| Rheumatology International | The presence of FCGR2B promoter or transmembrane region variant alleles leads to reduced serum IL-6 levels in rheumatoid arthritis[Pubmed:25630523] |
| The Journal of Biological Chemistry | 5-azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival[Pubmed:25691576] |
| Scandinavian Journal of Clinical and Laboratory Investigation | Osteocartilaginous metabolic markers change over a 3-week stage race in pro-cyclists[PubMed: 26174975] |
| AIDS | Effects of renal tubular dysfunction on bone in tenofovir-exposed HIV-positive patients[PubMed: 26372384] |
| the journal of rheumatology | Biomarkers in Remission According to Different Criteria in Patients with Rheumatoid Arthritis[PubMed: 26472417] |
| Int J Mol Med | Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway[PubMed: 26677102] |
| the journal of biological chemistry | Mutation in Osteoactivin Promotes RANKL-Mediated Osteoclast Differentiation and Survival, but Inhibits Osteoclast Function[PubMed: 25837253] |
| frontiers in endocrinology | Oxytocin reverses osteoporosis in a sex-dependent manner[PubMed: 26042090] |
| PLoS One. | The RIP1–RIP3 Complex Mediates Osteocyte Necroptosis after Ovariectomy in Rats[pmc:PMC4795547] |
| J Cell Physiol | Lanthanum Chloride Attenuates Osteoclast Formation and Function Via the DownreCavia (Guinea pig )lation of Rankl-Induced Nf-κb and Nfatc1 Activities[Pubmed:26060084] |
| FASEB Journal | N-Homocysteinylation impairs collagen cross-linking in cystathionine β-synthase-deficient mice: a novel mechanism of connective tissue abnormalities.[pubmed:27530978] |
| Metabolism. | High FGF21 levels are associated with altered bone homeostasis in HIV-1-infected patients.[pubmed:28521869] |
| journal of science and medicine in sport | The effect of calcium and vitamin D supplementation on bone health of male Jockeys.[pubmed:27568072] |
| Chemico-Biological Interactions | Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway[pubmed:27387537] |
| Epilepsy Research | The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats[pubmed:27838501] |
| Bone | Reduced femoral bone mass in both diet-induced and genetic hyperlipidemia mice[pubmed:27669658] |
| Journal of Vascular Surgery | A potential role for glycated cross-links in abdominal aortic aneurysm disease.[pubmed:27624703] |
| Institute of Pharmacology | Effects of long-term administration of pantoprazole on bone mineral density in young male rats.[pubmed:27552060] |
| Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats[S0753332216322752] |
| European Journal of Pharmacology | Cannabidiol administration reduces sublesional cancellous bone loss in rats with severe spinal cord injury[pubmed:28479140] |
| APPLIED SPECTROSCOPY | Side-Effects of Convulsive Seizures and Anti-Seizure Therapy on Bone in a Rat Model of Epilepsy[pubmed:28905646] |
| Clinical Nutrition | Obesity status influences the relationship among serum osteocalcin, iron stores and insulin sensitivity[pubmed:29050649] |
| Oncotarget | A novel osteoporosis model with ascorbic acid deficiency in Akr1A1 gene knockout mice.[pubmed:28060768] |
| 中国骨质疏松杂志 | 骨形成指标PINP 与骨吸收指标β-CTX 在2 型糖尿病合并骨质疏松中作用的研究.[10.3969/j.issn.1006-7108.2017.03.009] |
| BioMed Research International | Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research[pubmed:29119115] |
| Cell Death And Differentiation | TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42[Pubmed:29358671] |
| Journal of Periodontology | The role of androgens on periodontal repair in female rats[Pubmed:29683499] |
| Life Sciences | Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway[Pubmed:29883721] |
| European Journal of Pharmacology | The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism[Pubmed:29428468] |
| Journal of Clinical Densitometry | Geometric and mechanical bone response to a multidisciplinary weight loss intervention in adolescents with obesity: The ADIBOX study[Pubmed:30076009] |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats[Pubmed:29898457] |
| Experimental and Therapeutic Medicine | Liraglutide exerts a bone‑protective effect in ovariectomized rats with streptozotocin‑induced diabetes by inhibiting osteoclastogenesis[Pubmed:29805533] |
| Experimental and Therapeutic Medicine | Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice[10.3892:etm.2018.6353] |
| Tumor Biology | Dual PI3 K/mTOR inhibition reduces prostate cancer bone engraftment altering tumor-induced bone remodeling[Pubmed:29687745] |
| Sensors | Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related Biomarkers[Pubmed: 30181433] |
| Tropical Journal of Pharmaceutical Research | Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway[] |
| Bone & Joint Research | Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise[] |
| Toxicol Ind Health | Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed …[Pubmed: 30983556] |
| Journal of Geriatric Oncology | Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors[Pubmed: 30952517] |
| Scientific Reports | Significance of the Tks4 scaffold protein in bone tissue homeostasis[] |
| E The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats[] | |
| Biology Open | Osteocyte TSC1 promotes sclerostin secretion to restrain osteogenesis in mice[Pubmed: 31088250] |
| Journal of Periodontology | Role of testosterone and androgen receptor in periodontal disease progression in female rats[Pubmed: 31389012] |
| Journal of clinical periodontology | Biomarkers of oral inflammation in perinatally HIV‐infected and perinatally HIV exposed, uninfected youth[Pubmed: 31385616] |
| Bulletin of Experimental Biology and Medicine | Laboratory Monitoring of Bone Tissue Remodeling after Augmentation of Impression Intraarticular Fracture with Different Types of Bone Graft[Pubmed: 31630302] |
| Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities[Pubmed: 31739209] |
| International Journal of Environmental Research and Public Health | Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats[Pubmed: 31739465] |
| International journal of molecular sciences | Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients[Pubmed: 31717649] |
| Brazilian Journal of Medical and Biological Research | Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signals[Pubmed: 31826180] |
| Journal of Bone and Mineral Research | BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodeling[Pubmed: 31914211] |
| Theranostics | EDTA-Modified 17β-Estradiol-Laden Upconversion Nanocomposite for Bone-Targeted Hormone Replacement Therapy for Osteoporosis[Pubmed: 32194868] |
| Cells | Article Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous …[Pubmed: 31877673] |
| Cell | RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis[Pubmed: 32640190] |
| Advanced Science | A Loop‐Based and AGO‐Incorporated Virtual Screening Model Targeting AGO‐Mediated miRNA–mRNA Interactions for Drug Discovery to Rescue Bone Phenotype …[Pubmed: 32670749] |
| International Medical Journal Malaysia | The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats.[] |
| Cells. | Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous …[] |
| Journal of Orthopaedic Translation | Qu Feng Zhi Tong capsule increases mechanical properties of cortical bone in ovariectomized rats[] |
| Research square | 4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized Mice[] |
| IIUM Medical Journal Malaysia | The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats[] |
| 淫羊藿水提物及提取药渣的抗骨质疏松药效比较研究[] | |
| Pamukkale Tıp Dergisi | Postmenapozal Tip-2 diyabetes mellitus' lu hastalarda osteoporoza bağlı kırık riskinin ve kemik Turnoveri'nin değerlendirilmesi[] |
| Science Advances | A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair[33310848] |
| J Bone Miner Res | Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathway[33950533] |
| Br J Pharmacol | Andrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesis[34233019] |
| PHARMACEUTICAL BIOLOGY | Effects of icariin on the fracture healing in young and old rats and its mechanism[34511043] |
| Evid Based Complement Alternat Med | Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway[34512780] |
| Scandinavian Cardiovascular Journal | Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy[34296634] |
| Chronobiol Int | Night shift work and osteoporosis-bone turnover markers among female blue-collar workers in Poland[Pubmed:35139709] |
| Cell Metab | Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression[Pubmed:35235775] |
| Cell Death Discov | BMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axis[Pubmed:35523787] |
| Experimental and Molecular Medicine | Foxf2 represses bone formation via Wnt2b/β-catenin signaling[Pubmed:35668101] |
| Bioactive Materials | Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles[Pubmed:35386445] |
| Journal of Orthopaedic Translation | Puerarin specifically disrupts osteoclast activation via blocking integrin-β3 Pyk2/Src/Cbl signaling pathway[Pubmed:35228997] |
| Journal of Orthopaedic Translation | Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35 …[] |