肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Hepatocyte Growth Factor (HGF) ,etc. by FLIA (Flow Luminescence Immunoassay)
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
（注：单次混测多因子不超过8个指标 ）
- 编号LMA047Hu
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围1.95-2000pg/mL
- 灵敏度最小可检测剂量小于等于0.65 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格8指标数7指标数6指标数5指标数4指标数3指标数2指标数1指标数
- 价格¥ 2090¥ 2171¥ 2291¥ 2452¥ 2613¥ 2854¥ 3216¥ 4020 添加到价格计算器 计算器
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 82-96 | 91 |
| EDTA plasma(n=5) | 83-102 | 90 |
| heparin plasma(n=5) | 87-101 | 97 |
| sodium citrate plasma(n=5) | 88-96 | 93 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 83-101% | 97-104% | 80-104% |
| EDTA plasma(n=5) | 95-102% | 89-96% | 94-105% | 89-98% |
| heparin plasma(n=5) | 96-105% | 78-96% | 89-105% | 98-105% |
| sodium citrate plasma(n=5) | 79-101% | 82-91% | 90-98% | 89-96% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA047Hu03 | 肝细胞生长因子(HGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA047Hu01 | 肝细胞生长因子(HGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA047Hu02 | 肝细胞生长因子(HGF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA047Hu01 | 肝细胞生长因子(HGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA047Hu02 | 肝细胞生长因子(HGF)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA047Hu03 | 肝细胞生长因子(HGF)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA047Hu71 | 肝细胞生长因子(HGF)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA047Hu53 | 肝细胞生长因子(HGF)多克隆抗体(别藻蓝蛋白标记) | WB; IHC; ICC; IF. |
| LAA047Hu81 | 肝细胞生长因子(HGF)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA047Hu22 | 肝细胞生长因子(HGF)单克隆抗体 | WB; IHC |
| RAA047Hu21 | 肝细胞生长因子(HGF)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAA047Hu23 | 肝细胞生长因子(HGF)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA047Hu | 肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA047Hu | 肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA047Hu | 肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA047Hu | 肝细胞生长因子(HGF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA047Hu01 | 肝细胞生长因子(HGF)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Gene Therapy and Molecular Biology | Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heart[LWW: 01000] |
| Journal of Translational Medicine | Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma[BioMed: 14795876] |
| Digestive Diseases and Sciences | Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure[SpringerLink: k22823l138x2344x] |
| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children[PubMed: PMC3694825] |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)[PubMed: PMC3762604] |
| BMC cell biology | BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause[PubMed: PMC3640998] |
| Cell Journal (Yakhteh) | Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis[PubMed: PMC3584430] |
| Gastroenterol Res Pract | Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis[PubMed: 26448742] |
| Tumor Biology | Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma[PubMed: 26695151] |
| Cytotherapy | Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury[PubMed: 25863963] |
| Biomedicine & Pharmacotherapy | Silymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats[Pubmed:27261582] |
| Can J Physiol Pharmacol. | Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis[Pubmed:27186801] |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells[Pubmed:27323064] |
| Changes in HGF levels after a progressive test before and after Tabata HIIT program[profile:Pawel_Wolanski3] | |
| Stem Cell Research & Therapy | Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats[10.1186/s13287-017-0739-3] |
| Oncology Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c‑Met/PI3K/AKT in vitro[10.3892/ol.2017.7301] |
| Stem Cell Research & Therapy | Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure.[pubmed:28679425] |
| Nature Communications | Pulmonary pericytes regulate lung morphogenesis[Pubmed:29934496] |
| Experimental And Molecular Medicine | Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke …[Pubmed:29650950] |
| Oncology Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro[Pubmed:29375714] |
| Bulletin of Experimental Biology and Medicine | Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in Rats[Pubmed:29796817] |
| Cell Biology International | Inherent control of hepatocyte proliferationInherent control of hepatocyte proliferation after subtotal liver resection[Pubmed: 31297922] |
| Journal of stem cells & regenerative medicine | What is the Best Degree of Hyaluronic Acid Crosslinking in Increasing Growth Factors Level of Platelet-Rich Fibrin Lysate?[Pubmed: 31239605] |
| Open Journal of Veterinary Medicine | Efficacy of autologous bone marrow mononuclear cell transplantation in dogs with chronic spinal cord injury[Pubmed: 32821665] |
| Stem Cell Reviews and Reports | Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care[33469783] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …[Pubmed:35585377] |