细胞间粘附分子1(ICAM1)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Intercellular Adhesion Molecule 1 (ICAM1) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor
（注：单次混测多因子不超过8个指标 ）
- 编号LMA548Po
- 物种Sus scrofa; Porcine (Pig，猪)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.1-100ng/mL
- 灵敏度最小可检测剂量小于等于0.033 ng/mL.
- 样本类型serum, plasma, tissue homogenates and other biological fluids
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测细胞间粘附分子1(ICAM1)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的细胞间粘附分子1(ICAM1)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 91-99 | 96 |
| EDTA plasma(n=5) | 79-102 | 85 |
| heparin plasma(n=5) | 93-101 | 96 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的细胞间粘附分子1(ICAM1)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中细胞间粘附分子1(ICAM1)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-102% | 98-105% | 89-98% | 85-101% |
| EDTA plasma(n=5) | 96-104% | 94-105% | 99-105% | 99-105% |
| heparin plasma(n=5) | 89-103% | 94-101% | 86-96% | 95-103% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将细胞间粘附分子1(ICAM1)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的细胞间粘附分子1(ICAM1)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的细胞间粘附分子1(ICAM1)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的细胞间粘附分子1(ICAM1)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Sus scrofa; Porcine (Pig，猪) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA548Po01 | 细胞间粘附分子1(ICAM1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA548Po01 | 细胞间粘附分子1(ICAM1)多克隆抗体 | WB; IHC |
| LAA548Po81 | 细胞间粘附分子1(ICAM1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA548Po21 | 细胞间粘附分子1(ICAM1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA548Po | 细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA548Po | 细胞间粘附分子1(ICAM1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Amran et al. Lipids in Health and Disease | Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental.[BioMed: 1476511X] |
| Evidence-based Complementary and Alternative Medicine | Preventive Effects of a Natural Anti-Inflammatory Agent, Astragaloside IV, on Ischemic Acute Kidney Injury in Rats[PubMed: PMC3703719] |
| British Journal of Medical and Health Sciences | EFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATS[Researchgate: Source] |
| Cytokine | Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression.[Pubmed: 23434274] |
| BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations[Pubmed: 24199192] |
| Journal of Veterinary Internal Medicine | Markers of coagulation activation, endothelial stimulation, and inflammation in dogs with babesiosis[Pubmed: 23875771] |
| Arch Dis Child | PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) Newborns[Bmj:Source] |
| Arch Dis Child? | PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal Outcome[Bmj:Source] |
| Arch Dis Child | PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using Videography[Bmj:Source] |
| J Thorac Dis.? | Anti-endothelial cell antibodies in connective tissue diseases associated with pulmonary arterial hypertension[Pubmed:Pmc4015009] |
| Journal of Immunotoxicology | Acacia ferruginea inhibits cyclophosphamide-induced immunosuppression and urotoxicity by modulating cytokines in mice[Pubmed:24873678] |
| Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis[Pubmed:24992835] |
| Int J Neurosci. | The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits[Pubmed:25111247] |
| Naunyn-Schmiedeberg's Archives of Pharmacology | Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbits[Pubmed:25172523] |
| Nutr Res. | Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits[Pubmed:25282128] |
| Experimental & Molecular Medicine | Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns[Pubmed:25358349] |
| Neural Regen Res.? | Inflammatory response and neuronal necrosis in rats with cerebral ischemia[Pubmed:25422636] |
| Chemical Research in Toxicology | Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation[Pubmed:25580880] |
| CNS Neuroscience & Therapeutics | Peroxisome Proliferator-activated Receptor-γ Agonist Pioglitazone Ameliorates White Matter Lesion and Cognitive Impairment in Hypertensive Rats[Pubmed:25611692] |
| Alexandria Journal of Medicine | Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats[Sciencedirect:S2090506814000268] |
| Canadian Journal of Physiology and Pharmacology | Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism[Doi: Abs] |
| J Cell Biochem | Tumor Necrosis Factor-Alpha Up-Regulates ICAM-1 Expression and Release in Intestinal Myofibroblasts by Redox-Dependent and -Independent Mechanisms[PubMed: 26177712] |
| Neurotoxicology | Allopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in rats[PubMed: 25845300] |
| Alexandria Journal of Medicine | Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in[Science: Article] |
| Chemical Research in Toxicology | Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation[PubMed: 25580880] |
| Veterinary Parasitology | Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis[PubMed: 26412140] |
| Experimental Eye Research | The effect of triamcinolone acetonide or bevacizumab on the levels of proinflammatory cytokines after retinal laser photocoaCavia (Guinea pig )lation in pigmented rabbits[Pubmed:27296072] |
| Chinese Chemical Letters | Screening the anti-gout traditional herbs from TCM using an in vitro method[science:S1001841716301450] |
| Am J Physiol Lung Cell Mol Physiol | Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosis[Pubmed:26993522] |
| Mol Neurobiol | A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats[Pubmed:26867655] |
| Comparative Clinical Pathology | Identification of some plasma biomarkers associated with early weaning stress in crossbred piglets[article:10.1007] |
| Journal of Cellular Biochemistry | Tumor Necrosis Factor‐Alpha Up‐Regulates ICAM‐1 Expression and Release in Intestinal Myofibroblasts by Redox‐Dependent and‐Independent Mechanisms[pubmed:26177712] |
| BMC Vet Res. | Blood markers of fibrinolysis and endothelial activation in canine babesiosis.[pubmed:28363279] |
| Biomedicine & pharmacotherapy= Biomedecine & pharmacotherapie | Protective effects of N-acetylcystein and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusion[pubmed:28273631] |
| Pakistan journal of pharmaceutical sciences | Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil.[pubmed:29084680] |
| Experimental Parasitology | Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis[pubmed:28821422] |
| Molecular Medicine Reports | Age‑related differences in serum MFG‑E8, TGF‑β1 and correlation to the severity of atherosclerosis determined by ultrasound.[pubmed:29152651] |
| Journal of Microbiology, Immunology and Infection | Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients[pubmed:28655573] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis[S0021967317311858] |
| International Journal of Clinical and Experimental Pathology | Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo study[ISSN:1936-2625/IJCEP0055881] |
| Oncotarget | Global gene expression profile of cerebral ischemia-reperfusion injury in rat MCAO model[pubmed:29088811] |
| Microbial Pathogenesis | Protective effects of sinomenine against LPS-induced inflammation in piglets.[pubmed:28757275] |
| Journal of ophthalmology | Panretinal Photocoagulation Using Short-Pulse Laser Induces Less Inflammation and Macular Thickening in Patients with Diabetic Retinopathy[pubmed:28761761] |
| The Journal of Biological Chemistry | Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice[Pubmed:29929978] |
| Life Sciences | Lipoxin A4 may attenuate the progression of obesity-related glomerulopathy by inhibiting NF-κB and ERK/p38 MAPK-dependent inflammation[Pubmed:29499280] |
| International Journal of Molecular Medicine | Aspirin modulates the inflammatory response in a thrombus‑stimulated LMVEC model[Pubmed:29568915] |
| Clinical And Experimental Pharmacology And Physiology | Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway[Pubmed:29266336] |
| Medicine (Baltimore) | Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity[Pubmed:29953010] |
| Bioscience Reports | N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation.[Doi: 10.1042/BSR20180858] |
| Prostaglandins & Other Lipid Mediators | Detoxification and activating blood circulation decoction reduces restenosis involving the TLR4/NF-κB pathway after balloon injury[Pubmed: 30453089] |
| Biochemical and Biophysical Research Communications | The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusion[Pubmed: 30558792] |
| Nutrition & Metabolism | L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women[Pubmed: 30485835] |
| Cytokine | Intercellular adhesion molecule-1 (ICAM-1) associates with 24-hour ambulatory blood pressure variability in type 2 diabetes and controls[Pubmed: 30716657] |
| European Journal of Pharmacology | The cordycepin derivative IMM-H007 improves endothelial dysfunction by suppressing vascular inflammation and promoting AMPK-dependent eNOS activation in …[Pubmed: 30826323] |
| International journal of molecular sciences | Cellular Metabolomics Reveal the Mechanism Underlying the Anti-Atherosclerotic Effects of Aspirin Eugenol Ester on Vascular Endothelial Dysfunction[Pubmed: 31261711] |
| Experimental cell research | Resveratrol decreases TNFα-induced ICAM-1 expression and release by Sirt-1-independent mechanism in intestinal myofibroblasts[Pubmed: 31233740] |
| international journal of molecular sciences | Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells[Pubmed: 31671764] |
| Archives of Insect Biochemistry and Physiology | Vanadyl sulphate ameliorates biomarkers of endothelial injury and coagulation and thrombosis in a rat model of hyperglycaemia[Pubmed: 31774317] |
| BMC Endocrine Disorders | The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children[Pubmed: 31771561] |
| Scientific Reports | Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea[Pubmed: 31882827] |
| Journal of Applied Microbiology | The effects of dietary sodium butyrate supplementation on the growth performance, carcass traits, and intestinal microbiota of growing‐finishing pigs[Pubmed: 32048746] |
| LIFE SCIENCES | Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stress[Pubmed: 33227274] |
| Metab Brain Dis | Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in rats[Pubmed: 33170419] |
| BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | Endothelial epithelial sodium channel involves in high-fat diet-induced atherosclerosis in low-density lipoprotein receptor-deficient mice[Pubmed: 33065235] |
| BRITISH JOURNAL OF NUTRITION | Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats[Pubmed: 33028437] |
| MOLECULAR AND CELLULAR BIOCHEMISTRY | MicroRNA-217 ameliorates inflammatory damage of endothelial cells induced by oxidized LDL by targeting EGR1[Pubmed: 32737769] |
| OBESITY SURGERY | The Protective Effects of Butorphanol on Pulmonary Function of Patients with Obesity Undergoing Laparoscopic Bariatric Surgery: a Double-Blind Randomized …[Pubmed: 32535786] |
| Inflammation | Tetramethylpyrazine Inhibits Platelet Adhesion and Inflammatory Response in Vascular Endothelial Cells by Inhibiting P38 MAPK and NF-κB Signaling Pathways[] |
| J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans[33407555] |
| Molecules | Alpha-Lipoic Acid Plays a Role in Endometriosis: New Evidence on Inflammasome-Mediated Interleukin Production, Cellular Adhesion and Invasion[33430011] |
| Antioxidants (Basel) | Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model[33562069] |
| J Vet Intern Med | Association of markers of endothelial activation and dysfunction with occurrence and outcome of pulmonary hemorrhage in dogs with leptospirosis[34076314] |
| PLoS One | A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and …[34492054] |
| Sci. Pharm | Dehydroepiandrosterone (DHEA) Improves the Metabolic and Haemostatic Disturbances in Rats with Male Hypogonadism[] |
| Journal of the South African Veterinary Association | Circulating markers of endothelial activation in canine parvoviral enteritis[] |