白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Interleukin 17 (IL17) ,etc. by FLIA (Flow Luminescence Immunoassay)
IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
（注：单次混测多因子不超过8个指标 ）
- 编号LMA063Ca
- 物种Canis familiaris; Canine (Dog，犬)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.98-1000pg/mL
- 灵敏度最小可检测剂量小于等于0.327 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 79-104 | 88 |
| EDTA plasma(n=5) | 78-96 | 83 |
| heparin plasma(n=5) | 88-101 | 96 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-96% | 80-99% | 97-105% | 79-101% |
| EDTA plasma(n=5) | 84-93% | 89-102% | 84-97% | 90-104% |
| heparin plasma(n=5) | 93-102% | 84-101% | 80-104% | 88-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的白介素17(IL17)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Canis familiaris; Canine (Dog，犬) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA063Ca01 | 白介素17(IL17)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA063Ca01 | 白介素17(IL17)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA063Ca21 | 白介素17(IL17)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA063Ca | 白介素17(IL17)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA063Ca | 白介素17(IL17)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| PLOS ONE | Impaired Autoimmune T Helper 17 Cell Responses Following DNA Vaccination against Rat Experimental Autoimmune Encephalomyelitis[PubMed: PMC2577303] |
| Cellular Immunology | Atorvastatin ameliorates experimental autoimmune neuritis by decreased Th1/Th17 cytokines and up-regulated T regulatory cells[ScienceDirect: S0008874911002164] |
| The Journal of Urology | Improvement by Phytotherapeutic Agent of Detrusor Overactivity, Down-Regulation of Pharmacological Receptors and Urinary Cytokines in Rats with Cyclophosphamide Induced Cystitis[PubMed: S0022534712049087] |
| Critical Care Medicine | Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage[PubMed: PMC3383056] |
| Lower Urinary Tract Symptoms | Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide-Induced Cystitis[Onlinelibrary: luts.12015] |
| Shock | Expression of Hemopexin in Acute Rejection of Rat Liver Allograft Identified by Serum Proteomic Analysis.[Lww: Source] |
| J Vet Med Sci. | CD4+ T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel Disease[Pubmed:24270804] |
| Journal of Dairy Science | Increased blood-circulating interferon-γ, interleukin-17, and osteopontin levels in bovine paratuberculosis[Pubmed:24679935] |
| Inflammation.? | Pemetrexed Ameliorates Experimental Arthritis in Rats[Pubmed:25129058] |
| Inflammation | Lapatinib Ameliorates Experimental Arthritis in Rats[Pubmed:25283886] |
| Molecular and Cellular Biochemistry | Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide[Pubmed:25318610] |
| Molecular Medicine Reports | Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis[Pubmed:25323532] |
| Genet Mol Res | Protective effect and mechanism of probucol in the treatment of spinal cord injury[PubMed: 26214485] |
| veterinary research forum | Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells[Article_14936_6.Html: ] |
| Tumor Biology | Hypoxia-induced secretion of IL-20 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma[PubMed: 26695151] |
| Clinical & Experimental Immunology | MicroRNA‐155 may be involved in the pathogenesis of atopic dermatitis by modulating the differentiation and function of T helper type 17 (Th18) cells[PubMed: 25761610] |
| J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells[PubMed: 25799173] |
| Biomarkers | Interleukin-17 levels correlate with poor prognosis and vascular endothelial growth factor concentration in the serum of patients with non-small cell lung cancer[PubMed: 26301881] |
| Molecular Medicine Reports | Tanshinone IIA attenuates experimental autoimmune encephalomyelitis in rats[Pubmed:27357729] |
| SpringerPlus | Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway.[Pubmed:27026909] |
| International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery[Pubmed:26802602] |
| Journal of Agricultural Biotechnology | Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells[10610] |
| Veterinary Research Communications | Immunological and pathological investigations in equine experimental uveitis[pubmed:27344152] |
| Journal of Neuroinflammation | Th17-skewed immune response and cluster of differentiation 40 ligand expression in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.[pubmed:28114998] |
| American Journal of Translational Research | Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathology[pubmed:27830033] |
| Springerplus | Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway[pubmed:27026909] |
| Journal of Virology | Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys.[pubmed:] |
| J Nephropathol | Relationship between IL-17 serum level and ambulatory blood pressure in women with polycystic ovary syndrome[pubmed:28042549] |
| Journal of Nephropathology | Relationship between IL-17 and ambulatory blood pressure in polycystic ovary syndrome[8b168a09f5832df1b5c2f48631612458b2d8.pdf] |
| Basic Clin Pharmacol Toxicol. | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling.[pubmed:28032440] |
| Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice[pubmed:28250847] |
| Procalcitonin und proinflammatorische Zytokine in der bronchoalveolären Lavage beim lungenkranken Pferd unter inhalativer Glukokortikoidtherapie[FUDISS_thesis_000000103830] | |
| Scientific Report | Anti-asthmatic efect of pitavastatin through aerosol inhalation is associated with CD4+ CD25+ Foxp3+ T cells in an asthma mouse model[pubmed:28729731] |
| Basic & Clinical Pharmacology & Toxicology | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling[doi:10.1111] |
| Academic Journal | Antiinflammatory and antioxidant effects of gemcitabine in collagen-induced arthritis model[pubmed:28618762] |
| International Journal of Clinical and Experimental Pathology | Low-density neutrophils in severe fever with thrombocytopenia syndrome (SFTS) display decreased function to phagocytose SFTS virus and enhanced capacity to synthesize cytokines[ISSN:1936-2625/IJCEP0045334] |
| Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice[pubmed:28122397] |
| MYOPAIN | Increased Levels of IL-17, IL-23, MIP-1α, MCP-1 and Global Leukocytes in Fibromyalgia Patients[10.1080/24708593.2017.1357664] |
| Molecular Medicine Reports | Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia.[pubmed:29257310] |
| Molecular Medicine Reports | Interleukin-17 antagonist attenuates lung inflammation through inhibition of the ERK1/2 and NF-κB pathway in LPS-induced acute lung injury[10.3892/mmr.2017.6837] |
| ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis[pubmed:28608724] |
| International Immunopharmacology | Therapeutic effect of bone marrow mesenchymal stem cells pretreated with acetylsalicylic acid on experimental periodontitis in rats[pubmed:29195233] |
| Biomedical Research | Associations among interleukin-17, interferon-gamma, and acute coronary syndrome[ISSN 0970-938X] |
| Cellular Physiology and Biochemistry | Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory …[Pubmed:29672286] |
| Frontiers in Pharmacology | Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal …[Pubmed:29375374] |
| Bioscience Reports | Decreased miR-128 expression alleviated the progression of Rheumatoid arthritis by up-regulating the expression of TNFAIP3[Pubmed:29853534] |
| Medical Science Monitor | Involvement of T-Helper 9 Activation in a Mouse Model of Allergic Rhinitis[Pubmed:29982264] |
| Journal of International Medical Research | Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease[Pubmed:29950127] |
| Canadian Journal of Physiology and Pharmacology | Inhibition of microRNA-155 attenuates concanavalin A-induced autoimmune hepatitis by regulating Treg/Th17 cell differentiation[Pubmed: 30290123] |
| BMC Microbiology | Nystatin enhances the immune response against Candida albicans and protects the ultrastructure of the vaginal epithelium in a rat model of vulvovaginal …[Pubmed: 30359236] |
| Biology of Reproduction | Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathway[Pubmed: 30496353] |
| BMC MICROBIOLOGY | Enhanced antifungal activity of bovine lactoferrin-producing probiotic Lactobacillus casei in the murine model of vulvovaginal candidiasis[Pubmed: 30621597] |
| CNS Neuroscience & Therapeutics | Negative regulation of glial Tim‐3 inhibits the secretion of inflammatory factors and modulates microglia to antiinflammatory phenotype after experimental intracerebral?…[Pubmed: 30677253] |
| OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES | Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus[] |
| Frontiers in Microbiology | Local probiotic Lactobacillus crispatus and Lactobacillus delbrueckii exhibit strong antifungal effects against vulvovaginal candidiasis in a rat model[] |
| 中国免疫学杂志 | TRAF-6 调控TGF-β1-Smad2/Smad3 信号通路在Graves 病免疫发病机制中的作用[] |
| Microbial Pathogenesis | Therapeutic effectiveness of type I interferon in vulvovaginal candidiasis[Pubmed: 31158491] |
| Inflammation | Protein Kinase C Theta Inhibition Attenuates Lipopolysaccharide-Induced Acute Lung Injury through Notch Signaling Pathway via Suppressing Th17 Cell Response …[Pubmed: 31297750] |
| international immunopharmacology | Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice[Pubmed: 31228813] |
| Untersuchung einer Th17-vermittelten Immunantwort bei der kaninen idiopathischen Epilepsie[] | |
| Free Radical Biology and Medicine | Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in rat[Pubmed: 31255668] |
| BioMed Research International | Indoleamine 2, 3-Dioxygenase (IDO) Regulates Th17/Treg Immunity in Experimental IgA Nephropathy[] |
| Animals | Anti-Inflammatory State in Arabian Horses Introduced to the Endurance Training[Pubmed: 31462005] |
| World Journal of Pediatrics | Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis[Pubmed: 31641939] |
| Biotechnology and Applied Biochemistry | Epigallocatechin 3‐gallate attenuates arthritis by regulating Nrf‐2, HO‐1 and cytokine levels in an experimental arthritis model[Pubmed: 31746064] |
| Microbial Pathogenesis | Tangeretin attenuates lipopolysaccharide-induced acute lung injury through Notch signaling pathway via suppressing Th17 cell response in mice[Pubmed: 31676364] |
| International Journal of Rheumatic Diseases | Effects of thalidomide on Th17, Treg cells and TGF‐β1/Smad3 pathway in a mouse model of systemic sclerosis[Pubmed: 31840939] |
| Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis[Pubmed: 31927460] |
| international urology and nephrology | The therapeutic effect of dendritic cells expressing indoleamine 2, 3-dioxygenase (IDO) on an IgA nephropathy mouse model[Pubmed: 31894556] |
| JOURNAL OF PERIODONTAL RESEARCH | Salivary Del‐1, IL‐17, and LFA‐1 levels in periodontal health and disease[Pubmed: 32153040] |
| journal of molecular endocrinology | LncRNA Profile in Hashimoto's Thyroiditis and Potential Function of NONHSAT079547. 2[Pubmed: 32229697] |
| Toxicol Appl Pharmacol | Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling[Pubmed: 33171191] |
| CARDIOVASCULAR DRUGS AND THERAPY | Rhodiola crenulata reduces ventricular arrhythmia through mitigating the activation of IL-17 and inhibiting the MAPK signaling pathway[Pubmed: 32946036] |
| Biomed Research International | Effects of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis[Pubmed: 32908870] |
| J Neurosurg | Covering the proximal nerve stump with chondroitin sulfate proteoglycans prevents traumatic painful neuroma formation by blocking axon regeneration after …[Pubmed: 32470939] |
| JOURNAL OF MICROBIOLOGY | Melatonin attenuates microbiota dysbiosis of jejunum in short-term sleep deprived mice[Pubmed: 32424577] |
| bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat[Pubmed: 32395767] |
| Journal of Biochemical and Molecular Toxicology | Vitamin D3 and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti‐inflammatory actions by remodeling cellular …[] |
| Revista da Associacao Medica Brasileira | A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic …[] |
| biosciences biotechnology research asia | Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet.[] |
| ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction[] |
| Effects of minocycline on neuroinflammation, gut microbiome and hippocampal neurogenesis in rats with Gulf War Illness[] | |
| Minocycline Alleviate A Rat Model Of Gulf War Illness Via Altering Gut Microbiome, Attenuating Neuroinflammation And Enhancing Hippocampal Neurogenesis[] | |
| 中国应用生理学杂志 | 丹参对非酒精性脂肪肝Th17细胞及相关细胞因子的影响*[] |
| Rev Assoc Med Bras | A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?¡[33295414] |
| BEHAVIOURAL BRAIN RESEARCH | Minocycline alleviates Gulf War Illness rats via altering gut microbiome, attenuating neuroinflammation and enhancing hippocampal neurogenesis[34000339] |
| Int J Clin Pract | Serum Galectin©\3 Levels in Patients with Psoriasis[34137138] |
| cells | The Effect of the Clenbuterol¡ª¦Â2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen?¡[33920705] |
| Mediators of Inflammation | Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of ¦Á1, 3 ¡[] |
| CNS Neurosci Ther | Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke[34591349] |
| PLoS One | Evaluation of the involvement of Th17-cells in the pathogenesis of canine spinal cord injury[34591917] |
| J Periodontol | Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study[34730850] |
| Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive …[34802039] |
| Viruses | Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice[34960627] |
| PLoS One | Th17 cell-mediated immune response in a subpopulation of dogs with idiopathic epilepsy[Pubmed:35025939] |
| Pathogens | Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients[Pubmed:35056005] |
| Biomed Pharmacother | Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis[Pubmed:35453007] |
| npj Biofilms and Microbiomes | The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal[Pubmed:35379849] |
| Expert Opinion on Therapeutic Targets | Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma[Pubmed:35549595] |