武汉云克隆科技股份有限公司

白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Interleukin 33 (IL33) ,etc. by FLIA (Flow Luminescence Immunoassay)

DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules

（注：单次混测多因子不超过8个指标 ）

  • 白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)产品包装(模拟)
  • 白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
serum(n=5)87-9490
EDTA plasma(n=5)96-105101
heparin plasma(n=5)97-104101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本1:21:41:81:16
serum(n=5)94-105%81-101%79-91%78-101%
EDTA plasma(n=5)82-96%93-105%78-101%94-105%
heparin plasma(n=5)92-101%98-105%91-105%78-96%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
RPB980Hu02白介素33(IL33)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APB980Hu01白介素33(IL33)活性蛋白Cell culture; Activity Assays.
RPB980Hu01白介素33(IL33)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB980Hu02白介素33(IL33)多克隆抗体WB; IHC; ICC; IP.
PAB980Hu01白介素33(IL33)多克隆抗体WB; IHC; ICC; IP.
LAB980Hu71白介素33(IL33)多克隆抗体(生物素标记)WB; IHC; ICC.
RAB980Hu21白介素33(IL33)重组抗体WB; IF; ICC; IHC; IP; FCM.
MAB980Hu24白介素33(IL33)单克隆抗体WB; IHC; ICC; IP.
MAB980Hu26白介素33(IL33)单克隆抗体WB; IHC; ICC; IP.
MAB980Hu21白介素33(IL33)单克隆抗体WB; IHC; ICC; IP.
MAB980Hu22白介素33(IL33)单克隆抗体WB; IHC
MAB980Hu23白介素33(IL33)单克隆抗体WB; IHC; ICC; IP.
MAB980Hu25白介素33(IL33)单克隆抗体WB; IHC; ICC; IP.
LAB980Hu72白介素33(IL33)单克隆抗体(生物素标记)WB; IHC; ICC.
LAB980Hu76白介素33(IL33)单克隆抗体(生物素标记)WB; IHC; ICC.
SEB980Hu白介素33(IL33)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCB980Hu白介素33(IL33)检测试剂盒(化学发光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMB980Hu白介素33(IL33)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
PSB980Hu01白介素33(IL33)抗体对ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB980Hu01白介素33(IL33)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
The Korean Journal of Internal MedicineInterleukin-33, matrix metalloproteinase-9, and tissue ınhibitor of matrix metalloproteinase-1 in myocardial infarction[PubMed: PMC3604606]
Acta HistochemCXCL13 blockade attenuates lupus nephritis of MRL/lpr mice[PubMed: 26456520]
Journal of Interferon & Cytokine ResearchEpithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis[Pubmed:26540312]
Journal of Interferon & Cytokine ResearchThe Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway.[pubmed:27348082]
Archives of Dermatological ResearchIL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligo[pubmed:27388717]
Sci Pharm.Chrysin Protects Rat Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. [pubmed:28134775]
Biotechnology & Biotechnological Equipment Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese)[10.1080:13102818.2018.1471361]
Tropical Journal of Pharmaceutical ResearchRole of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer[172341]
Particle and Fibre ToxicologyGroup II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposure[Pubmed: 30413212]
Scientific ReportsInterleukin-33 serum levels in postmenopausal women with osteoporosis[Pubmed: 30846811]
Cell ReportsTumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s[Pubmed: 32101749]
Allergy Asthma & Immunology ResearchRelationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmatics[Pubmed: 32141256]
Traditional Medicine ResearchQingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …[]
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota[]
J Immunol ResInhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus[34395633]
PathogensElevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients[Pubmed:35056005]
researchsquareDexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling[]