白介素35(IL35)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Interleukin 35 (IL35) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：单次混测多因子不超过8个指标 ）
- 编号LMC008Mu
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.49-500pg/mL
- 灵敏度最小可检测剂量小于等于0.163 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测白介素35(IL35)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的白介素35(IL35)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-101 | 90 |
| EDTA plasma(n=5) | 78-101 | 82 |
| heparin plasma(n=5) | 87-97 | 94 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的白介素35(IL35)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中白介素35(IL35)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 89-96% | 78-101% | 83-99% |
| EDTA plasma(n=5) | 93-102% | 83-90% | 82-93% | 79-95% |
| heparin plasma(n=5) | 97-104% | 86-97% | 95-105% | 93-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将白介素35(IL35)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的白介素35(IL35)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的白介素35(IL35)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的白介素35(IL35)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC008Mu02 | 白介素35(IL35)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC008Mu01 | 白介素35(IL35)活性蛋白 | Cell culture; Activity Assays. |
| RPC008Mu01 | 白介素35(IL35)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC008Mu01 | 白介素35(IL35)多克隆抗体 | WB; IHC; ICC; IP. |
| SEC008Mu | 白介素35(IL35)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEC008Mu | 白介素35(IL35)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCC008Mu | 白介素35(IL35)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMC008Mu | 白介素35(IL35)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Oncology Letters | Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia.[PubMed: 22783403] |
| Journal of Maternal-Fetal and Neonatal Medicine | Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease[Pubmed: 24175856] |
| Journal of Reproductive Immunology | What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?[Pubmed: 24368037] |
| Human Immunology | The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia[ScienceDirect: S0198885913005776] |
| Folia Biologica (Praha) | Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers[Pubmed:24785110] |
| Molecular Medicine Reports | Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis[Pubmed:25323532] |
| Rom J Morphol Embryol. | The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy[Pubmed:25611266] |
| Journal of Immunotherapy | Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity[PubMed: 25962106] |
| PLOS ONE | Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis[PubMed: 26204444] |
| Rheumatology (Oxford) | Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease[PubMed: 26231346] |
| Cytokine | Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia[PubMed: 26282938] |
| J Biol Chem | Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway[PubMed: 26085094] |
| Br J Haematol | Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia[PubMed: 25640666] |
| Autoimmunity Reviews | New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literature[Pubmed:26876383] |
| Clinical research | Increased plasma concentrations of interleukin 35 in patients with coronary artery disease[Journal:-19] |
| Clinical Rheumatology | The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis[pubmed:27502600] |
| Respiratory Medicine | Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis[pubmed:27492538] |
| International Journal of Molecular Medicine | Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines.[pubmed:27460435] |
| 上海口腔医学 | IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析[QK:98604X] |
| Reproductive Sciences | Upregulation of Interleukin 35 in Patients With Endometriosis Stimulates Cell Proliferation[pubmed:28659009] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | IL‑35 may maintain homeostasis of the immune microenvironment in periodontitis[issn:2542-3975] |
| The Journal of Rheumatology | Synoviocytes-derived Interleukin 35 Potentiates B Cell Response in Patients with Osteoarthritis and Rheumatoid Arthritis[pubmed:29247146] |
| Cellular & Molecular Immunology | Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitro[pubmed:28713164] |
| Molecular Medicine Reports | Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia.[pubmed:29257310] |
| Asian pacific journal of allergy and immunology | Evaluation of serum interleukin- 35 level in children with persistent asthma.[pubmed:27996283] |
| PLoS One | Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio[pubmed:29236782] |
| Hepatology Communications | Significant association between FOXP3 gene polymorphism and steroid-resistant acute rejection in living donor liver transplantation[10.1002/hep4.1052] |
| International Journal of Clinical and Experimental Medicine | Clinical significance of IL-35 expression in the progression of clear cell renal cell carcinoma [ISSN:1940-5901] |
| OncoTargets and Therapy | Interleukin-35 as a predictor of prostate cancer in patients undergoing initial prostate biopsy.[pubmed:28761357] |
| Annals of Rheumatic Diseases | AB0734 Serum il-35 levels in systemic sclerosis and relationship with clinical features[10.1136:annrheumdis-2018-eular.2605] |
| Tohoku Journal of Experimental Medicine | Interleukin-35 as a New Biomarker of Renal Involvement in Lupus Nephritis Patients[Pubmed:29576585] |
| Turkish Journal Of Medical Sciences | The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility[Pubmed:29914245] |
| Oncotarget | Pro-tumoral immune cell alterations in wild type and Shb-deficient mice in response to 4T1 breast carcinomas[Pubmed:29721156] |
| Journal of Clinical Rheumatology | Serum Interleukin 35 Levels in Systemic Sclerosis and Relationship With Clinical Features.[Pubmed: 30431486] |
| Oncology Letters | Interleukin‑35 is associated with the tumorigenesis and progression of prostate cancer[] |
| Journal of Clinical Investigation | Plasmacytoid dendritic cells protect against immune-mediated acute liver injury via IL-35[Pubmed: 31264967] |
| Cells | CD4+ FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired[Pubmed: 32111105] |
| Cytokine | Interleukin-35 in idiopathic inflammatory myopathies[Pubmed: 33128920] |
| FEBS Open Bio | IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1[Pubmed: 33064893] |
| Cancer Cell International | Interleukin-35 promotes progression of prostate cancer and inhibits anti-tumour immunity[Pubmed: 33041668] |
| Journal of Neuroinflammation | Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain[Pubmed: 32375811] |
| Int Immunopharmacol | Association of IL-35 expression and gene polymorphisms in rheumatoid arthritis[33307515] |
| Archives of Medical Science | Decreased interleukin-35 levels and CD4+ EBI3+ T cells in patients with type 1 diabetes and the effects of the antibody against CD20 (rituximab)[33488880] |
| Asia Pac Allergy | Anti-inflammatory roles of interleukin-35 in the pathogenesis of Japanese cedar pollinosis[34386410] |
| Int Immunopharmacol | Pentosan Polysulfate Sodium augments the therapeutic effect of 5-Aminosalicylic Acid in DSS colitis model; the role of IL-35 expression[Pubmed:35247859] |
| Biology and Medical Researches | Association of three single nucleotide polymorphisms of interleukin-35 for detection and prediction of women with breast cancer in Erbil city[] |
| Research Square | Analysis of IL-10 and IL-35 in DPP-4 inhibitor-related bullous pemphigoid[] |