组织因子(TF)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III

(注:单次混测多因子不超过8个指标 )

特异性

本试剂盒用于检测组织因子(TF)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的组织因子(TF)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
EDTA plasma(n=5)92-10196
heparin plasma(n=5)95-10399

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的组织因子(TF)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中组织因子(TF)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本1:21:41:81:16
EDTA plasma(n=5)96-103%78-97%78-104%88-101%
heparin plasma(n=5)98-105%86-104%78-102%98-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
    37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将组织因子(TF)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的组织因子(TF)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的组织因子(TF)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的组织因子(TF)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号适用物种:Mus musculus (Mouse,小鼠)应用(仅供研究使用,不用于临床诊断!)
RPA524Mu02组织因子(TF)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA524Mu01组织因子(TF)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA524Mu01组织因子(TF)多克隆抗体WB; IHC; ICC; IP.
PAA524Mu02组织因子(TF)多克隆抗体WB; IHC; ICC; IP.
LAA524Mu71组织因子(TF)多克隆抗体(生物素标记)WB; IHC; ICC.
LAA524Mu81组织因子(TF)多克隆抗体(异硫氰酸荧光素标记)WB; IHC; ICC; IF.
MAA524Mu21组织因子(TF)单克隆抗体WB; IHC; ICC; IP.
SEA524Mu组织因子(TF)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA524Mu组织因子(TF)检测试剂盒(化学发光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA524Mu组织因子(TF)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

参考文献

杂志参考文献
PeptidesCalcitonin gene-related peptide-mediated antihypertensive and anti-platelet effects by rutaecarpine in spontaneously hypertensive rats[PubMed: 18625276]
Canadian Journal of Physiology and PharmacologyIncreased plasma level of asymmetric dimethylarginine in hypertensive rats facilitates platelet aggregation: role of plasma tissue factor[Nrcresearchpress: y10-115]
International immunopharmacologyRuscogenin attenuates monocrotaline-induced pulmonary hypertension in rats[Pubmed: 23538027]
BioMed research internationalEndothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations[Pubmed: 24199192]
The Journal of Biological ChemistrySphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy[Pubmed:25505239]
HippokratiaThrombosis in Hemodialysis Patients; Their Association with Tissue Factor and Tissue Factor Pathway Inhibitor[PubMed: 20011089]
shockNoble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock[PubMed: 25423126]
PerfusionEx vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing[PubMed: 26243277]
Molecular Nutrition & Food ResearchTrans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors[PubMed: 25546502]
Food FunctThe effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice[Pubmed:26757699]
Blood Cells, Inflammation and InfectionNebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury[pubmed:29202212]
Thrombosis andHaemostasisNebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndrome[Pubmed: 29202212]
Journal of Thrombosis and HaemostasisEffects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in rats[Pubmed: 31755229]
Mediators InflammAmelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB …[Pubmed: 32587471]
International Journal of Clinical PracticeEffect of Glucocorticoid Administration in Intravenous Pulses on Selected Parameters of the Coagulation System[Pubmed:35685509]