中期因子(MK)重组蛋白
Recombinant Midkine (MK)
MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2
- 编号RPA631Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位分泌
- 预测分子量18.9kDa
- 实际分子量18kDa(差异分析请参阅说明书)
- 片段与标签Ala22~Asp140 with N-terminal His Tag
- 缓冲液成份100mM NaHCO3, 500mM NaCl缓冲液（pH8.3,含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点9.3
- 应用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，请参见活性蛋白。 - 下载英文说明书 中文说明书
- 规格10µg50µg200µg1mg5mg
- 价格¥ 1008¥ 2520¥ 5040¥ 15120¥ 37800
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
Figure. SDS-PAGE
通过ISO 9001、ISO 13485质量体系认证
序列
用法
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA631Mu01 | 中期因子(MK)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA631Mu02 | 中期因子(MK)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA631Mu01 | 中期因子(MK)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA631Mu02 | 中期因子(MK)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA631Mu71 | 中期因子(MK)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA631Mu81 | 中期因子(MK)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| SEA631Mu | 中期因子(MK)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA631Mu | 中期因子(MK)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA631Mu01 | 中期因子(MK)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Midkine: From Embryogenesis to Pathogenesis and Therapy | Immunochemical Methods for the Detection of Midkine in Samples of Human Origin[Springer: Source] |
| 33 | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and …[10.1016:j.ebiom.2017.11.020] |
| Circulation Journal | Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice[pubmed:28781288] |
| EBioMedicine | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and …[Pubmed:29233575] |
| Journal of Clinical Laboratory Analysis | Clinical value of jointly detection pleural fluid Midkine, pleural fluid adenosine deaminase, and pleural fluid carbohydrate antigen 125 in the identification of nonsmall …[Pubmed:29797475] |
| Charakterisierung der funktionalen Rolle des Zytokins Midkine bei der Arteriogenese[:] | |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study[Pubmed: 32198440] |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells[Pubmed: 33206337] |