血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法,小样本)
Mini Samples ELISA Kit for Angiotensin 1-7 (Ang1-7)
- 编号MES085Mi
- 物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长3h
- 检测范围9.88-800pg/mL
- 灵敏度最小可检测剂量小于等于3.37pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 100% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples.
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 3914¥ 5592¥ 25164¥ 47532¥ 391440
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特异性
本试剂盒对检测血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法,小样本)具有较高的灵敏度。
经检测人、小鼠、大鼠之间有100%的交叉反映。
回收率
分别于定值血清及血浆样本中加入一定量的血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法,小样本)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 79-97 | 89 |
| EDTA plasma(n=5) | 79-105 | 82 |
| heparin plasma(n=5) | 78-95 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法,小样本)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 97-104% | 87-99% | 83-97% |
| EDTA plasma(n=5) | 90-102% | 96-104% | 91-101% | 80-90% |
| heparin plasma(n=5) | 92-101% | 94-101% | 87-104% | 78-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）25µL后，立即加入检测溶液A25µL，
37°C孵育1小时；
3. 甩干，洗板3次；
4. 加检测溶液B50µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分钟；
7. 加终止液25µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法,小样本)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| CPS085Mi21 | 血管紧张素1-7(Ang1-7)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPS085Mi11 | 血管紧张素1-7(Ang1-7)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAS085Mi08 | 血管紧张素1-7(Ang1-7)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAS085Mi27 | 血管紧张素1-7(Ang1-7)单克隆抗体 | WB; IHC; ICC; IP. |
| MAS085Mi22 | 血管紧张素1-7(Ang1-7)单克隆抗体 | IHC |
| MAS085Mi21 | 血管紧张素1-7(Ang1-7)单克隆抗体 | WB; IHC; ICC; IP. |
| MES085Mi | 血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CES085Mi | 血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMS085Mi | 血管紧张素1-7(Ang1-7)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| J Stroke Cerebrovasc Dis | Improvement of Plasma Biomarkers after Switching Stroke Patients from Other Angiotensin II Type I Receptor Blockers to Olmesartan[PubMed: 25891757] |
| Cellular Physiology and Biochemistry | Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats[pubmed:28848201] |
| Frontiers in Physiology | Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression[pubmed:29066981] |
| Molecular Medicine Reports | The ACE2-Ang (1-7)-Mas receptor axis attenuates cardiac remodeling and fibrosis in post-myocardial infarction.[pubmed:28656296] |
| Journal of Clinical Pharmacy and Therapeutics | Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites.[pubmed:28895159] |
| Molecular Medicine Reports | Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats[pubmed:28944898] |
| Open Journal of Endocrine and Metabolic Diseases Paper Submission | A Study of the Correlation between Angiotensin (1-7) and the Histopathological Changes in the Penises of Experimentally Diabetic Rats[2-1980272_83198.htm] |
| Journal of the American Society of Hypertension | Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system[Pubmed:29752040] |
| Innate Immunity | Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis[Pubmed:29969931] |
| Evidence-based Complementary and Alternative Medicine | Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects[Pubmed:29977313] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | The impact of ovulation inducing drugs on carcinogenesis[] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Changes of angiotensin II (1–8) and angiotensin (1–7) levels in early and late-onset preeclampsia[] |
| Journals of Gerontology Series A-Biological Sciences and Medical Sciences | Therapeutic Delivery of Ang (1-7) Via Genetically Modified Probiotic: A Dosing Study.[Pubmed: 31586210] |
| journal of the european academy of dermatology and venereology | Significance of the angiotensin I/angiotensin II/angiotensin‐(1‐7) axis in the pathogenesis of systemic sclerosis[Pubmed: 31746507] |
| Medicine | Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study[Pubmed: 32756181] |
| Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum …[] | |
| Amino Acids | Angiotensin-(1¨C7) attenuates collagen-induced arthritis via inhibiting oxidative stress in rats[33398523] |
| Autoimmunity | The prevalence and role of functional autoantibodies to angiotensin-converting-enzyme-2 in patients with systemic sclerosis[33910447] |
| researchsquare | Ang-(1-7) Protects Skeletal Muscle Function in Aged Mice[] |
| Peptides | Angiotensin-(1-7) protects against sepsis-associated left ventricular dysfunction induced by lipopolysaccharide[34298021] |
| BMC Musculoskeletal Disorders | Ang-(1–7) protects skeletal muscle function in aged mice[34548056] |
| J Cardiovasc Pharmacol | Resveratrol Confers Protection Against Ischemia/Reperfusion Injury by Increase of Angiotensin (1-7) Expression in a Rat Model of Myocardial Hypertrophy[34232225] |
