4-羟基苯丙酮酸双加氧酶(HPD)单克隆抗体
Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)
PPD; 4-HPPD; 4HPPD; GLOD3; Glyoxalase Domain Containing 3; 4-hydroxyphenylpyruvic acid oxidase
- 编号MAE251Hu21
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 来源单抗制备
- 宿主小鼠
- 效价(克隆号)D2
- Ig亚型 IgG2a Kappa
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- 免疫原RPE251Hu01-4-羟基苯丙酮酸双加氧酶(HPD)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度1mg/ml
- 且适物种Sus scrofa; Porcine (Pig，猪)
- 应用WB; IHC; ICC/IF
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载英文说明书 中文说明书
- 规格20µl100µl200µl1ml10ml
- 价格¥ 666¥ 1554¥ 2220¥ 5550¥ 22200
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产品包装(模拟)
产品包装(模拟)
DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 30µg/ml Mouse Anti-Human HPD Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human HPD Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 30µg/ml Mouse Anti-Human HPD Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 30µg/ml Mouse Anti-Human HPD Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Mouse Anti-Human HPD Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
Western Blot Sample: Lane1: Human Liver lysate; Lane2: Porcine Kidney lysate; Lane3: HepG2 cell lysate;<br/>Primary Ab: 2µg/ml Mouse Anti-Human HPD Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对HPD的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别HPD。
用法
Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-30µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPE251Hu01 | 4-羟基苯丙酮酸双加氧酶(HPD)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE251Hu01 | 4-羟基苯丙酮酸双加氧酶(HPD)多克隆抗体 | WB; IHC; ICC; IP. |
| MAE251Hu21 | 4-羟基苯丙酮酸双加氧酶(HPD)单克隆抗体 | WB; IHC; ICC/IF |
| MAE251Hu25 | 4-羟基苯丙酮酸双加氧酶(HPD)单克隆抗体 | WB; IHC; ICC; IP. |
| MAE251Hu23 | 4-羟基苯丙酮酸双加氧酶(HPD)单克隆抗体 | WB; IHC |
| MAE251Hu24 | 4-羟基苯丙酮酸双加氧酶(HPD)单克隆抗体 | WB; IHC; ICC; IP. |
| MAE251Hu22 | 4-羟基苯丙酮酸双加氧酶(HPD)单克隆抗体 | WB; IHC |
| MAE251Hu27 | 4-羟基苯丙酮酸双加氧酶(HPD)单克隆抗体 | WB; IHC; ICC; IP. |
| MAE251Hu29 | 4-羟基苯丙酮酸双加氧酶(HPD)单克隆抗体 | WB; IHC; ICC; IP. |
| FAE251Hu02 | 抗4-羟基苯丙酮酸双加氧酶(HPD)单克隆抗体 | Flow cytometry. |
| WEE251Hu | 4-羟基苯丙酮酸双加氧酶(HPD)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEE251Hu | 4-羟基苯丙酮酸双加氧酶(HPD)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME251Hu | 4-羟基苯丙酮酸双加氧酶(HPD)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Exp Gerontol. | Age associated decline in the conversion of leucine to β-Hydroxy-β-Methylbutyrate in rats[Pubmed:27073170] |