红细胞生成素(EPO)单克隆抗体
Monoclonal Antibody to Erythropoietin (EPO)
EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
- 编号MAA028Eq21
- 物种Equus caballus; Equine (Horse,马)相同的名称,不同的物种。
- 来源单抗制备
- 宿主小鼠
- 效价(克隆号)C3
- Ig亚型 IgG1 Kappa
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- 免疫原-
- 缓冲液成份0.01M 磷酸盐缓冲液(pH7.4,containing 0.05% Proclin-300,50% glycerol)
- 性状液体
- 浓度1mg/ml
- 且适物种-
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术,请参见流式抗体。 - 下载英文说明书 中文说明书
- 规格20µl100µl200µl1ml10ml
- 价格¥ 734 ¥ 1714¥ 2448¥ 6120¥ 24480
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
该抗体是针对EPO的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别EPO。
用法
Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
赠品
增值服务
相关产品
编号 | 适用物种:Equus caballus; Equine (Horse,马) | 应用(仅供研究使用,不用于临床诊断!) |
RPA028Eq01 | 红细胞生成素(EPO)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA028Eq01 | 红细胞生成素(EPO)多克隆抗体 | WB; IHC; ICC; IP. |
MAA028Eq21 | 红细胞生成素(EPO)单克隆抗体 | WB; IHC; ICC; IP. |
SEA028Eq | 红细胞生成素(EPO)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA028Eq | 红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
Endocrinology | Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat Offspring[Endo: source] |
Journal of Ethnopharmacology | Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression[PubMed: 21333724] |
PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation[PlosOne: Source] |
The Journal of Clinical Endocrinology & Metabolism | Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the Ovulation[PubMed: 22948762] |
Experimental & Molecular Medicine | Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression[PubMed: PMC3509184] |
Biochem Biophys Res Commun. | Reevaluation of erythropoietin production by the nephron[Pubmed:24832733] |
Nutrition. | Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m.[Pubmed:2528040] |
J Appl Physiol (1985). | Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness[Pubmed:25324511] |
Expert Rev Proteomics | Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone[PubMed: 26496240] |
Oncotarget | KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin[pubmed:26575329] |
international journal of molecular sciences | Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors.[pubmed:28216608] |
Veterinary Clinical Pathology | Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease[pubmed:28186645] |
IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …[articles:journal-of-alzheimers-disease] |
Oxidative Medicine and Cellular Longevity | Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation[pubmed:29147463] |
PLoS Neglected Tropical Diseases | Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis[pubmed:28489854] |
Neurochemistry International | Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia [S0197018617304783] |
Pesquisa Veterinária Brasileira | Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease[10.1590/s0100-736x2017000600011 ] |
Medical Journal Armed Forces India | Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation[10.1016/j.mjafi.2017.04.003] |
Neurochemistry International | Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on …[Pubmed:29180303] |
Clinical and Experimental Medicine | Increased NGAL level associated with iron store in chronic kidney disease with anemia[Pubmed:29909502] |
Bulletin of Experimental Biology and Medicine | Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia[Pubmed:29308565] |
Bulletin of Experimental Biology and Medicine | Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in …[Pubmed: 30353332] |
FASEB JOURNAL | High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans[] |
Journal of Functional Foods | Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats[] |
博士论文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE[] |
Nutrition | Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind …[Pubmed: 31743809] |
BRITISH JOURNAL OF HAEMATOLOGY | Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humans[Pubmed: 32866306] |
JCI Insight. | Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromes[Pubmed: 32759495] |
Blood Cells, Molecules, and Diseases | EPAS1 regulates proliferation of erythroblasts in chronic mountain sickness[Pubmed: 32470757] |
Int J Endocrinol | miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia[34367277] |
J Mater Sci Mater Med | Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules[Pubmed:35072831] |
Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells[] |