武汉云克隆科技股份有限公司

磷脂酰肌醇蛋白聚糖3(GPC3)单克隆抗体

Monoclonal Antibody to Glypican 3 (GPC3)

DGSX; OCI5; SDYS; SGB; SGBS1; MXR7; Glypican Proteoglycan 3; GTR2-2; Intestinal protein OCI-5; Secreted glypican-3

  • 磷脂酰肌醇蛋白聚糖3(GPC3)单克隆抗体产品包装(模拟)
  • 磷脂酰肌醇蛋白聚糖3(GPC3)单克隆抗体产品包装(模拟)
  • MAA971Hu24.jpgWestern Blot; Sample: Lane1: Human Placenta lysate; Lane2: Rat Liver lysate; Lane3: Rat Kidney lysate; Lane4: HepG2 cell lysate Primary Ab: 2µg/ml Mouse Anti-Human GPC3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对GPC3的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别GPC3。

用法

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

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编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
RPA971Hu02磷脂酰肌醇蛋白聚糖3(GPC3)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA971Hu02磷脂酰肌醇蛋白聚糖3(GPC3)活性蛋白Cell culture; Activity Assays.
RPA971Hu01磷脂酰肌醇蛋白聚糖3(GPC3)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA971Hu01磷脂酰肌醇蛋白聚糖3(GPC3)活性蛋白Cell culture; Activity Assays.
PAA971Hu01磷脂酰肌醇蛋白聚糖3(GPC3)多克隆抗体WB; IHC; ICC; IP.
MAA971Hu21磷脂酰肌醇蛋白聚糖3(GPC3)单克隆抗体WB; IHC; ICC; IP.
MAA971Hu22磷脂酰肌醇蛋白聚糖3(GPC3)单克隆抗体WB; IHC; ICC; IP.
MAA971Hu24磷脂酰肌醇蛋白聚糖3(GPC3)单克隆抗体WB; IHC; ICC; IP.
MAA971Hu23磷脂酰肌醇蛋白聚糖3(GPC3)单克隆抗体WB; IHC; ICC; IP.
MAA971Hu25磷脂酰肌醇蛋白聚糖3(GPC3)单克隆抗体WB; IHC; ICC; IP.
FAA971Hu82抗磷脂酰肌醇蛋白聚糖3(GPC3)单克隆抗体(异硫氰酸荧光素标记)Flow cytometry.
SEA971Hu磷脂酰肌醇蛋白聚糖3(GPC3)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA971Hu磷脂酰肌醇蛋白聚糖3(GPC3)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

参考文献

杂志参考文献
Nature and ScienceDiagnostic Role of Serum Glypican-3 as a Tumor Marker for Hepatocellular Carcinoma [Sciencepub:10043238]
Journal of Applied Pharmaceutical ScienceCytotoxic effects of antiglypican-3 against HepG2 cell line[Ebscohost: Source]
Asian Pacific Journal of Cancer PreventionCan Glypican3 be Diagnostic for Early Hepatocellular Carcinoma among Egyptian Patients?[Pubmed: 24460300]
Clin Biochem.Diagnostic accuracy of serum glypican-3 for hepatocellular carcinoma: A systematic review and meta-analysis[Pubmed: 24362268]
Science and NatureDiagnostic Role of Serum Glypican-3 as Hepatocellular Carcinoma[Zolcat: Source]
Alexandria Journal of MedicineRole of serum glypican-3 in the diagnosis and differentiation of small hepatocellular carcinoma from hepatitis-C virus cirrhosis[ScienceDirect: S2090506814000062]
J Egypt Natl Canc Inst.Comparing prothrombin induced by vitamin K absence-II (PIVKA-II) with the oncofetal proteins glypican-3, Alpha feto protein and carcinoembryonic antigen in diagnosing hepatocellular carcinoma among Egyptian patients.[Pubmed:24841158]
European Journal of PharmacologyEvaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma[Pubmed:25449037]
Hepatobiliary Pancreat Dis IntPredictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern[abstract:abstract4525.shtml]
Clinical and Molecular Hepatology Glypican-3 level assessed by the enzyme-linked immunosorbent assay is inferior to alpha-fetoprotein level for hepatocellular carcinoma diagnosis[pmc:articles]
WileyGlypican‐3: A promising biomarker for hepatocellular carcinoma diagnosis and treatment[pubmed:28621802]
Biomedicine & PharmacotherapyCytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and[Pubmed:29669302]
Journal of Cancer and Clinical OncologyDevelopment and Validation of a Two-Site Immunoradiometric assay for Glypican-3 in plasma: Implication in Diagnosis of Hepatocellular Carcinoma[Researchgate:Source]
Turkish journal of oncologySerum Glypican-3, Vascular Endothelial Growth Factor, and Interleukin-6 Levels in Hepatocellular Carcinoma[10.5505:tjo.2018.1742]
Intensive Care Medicine ExperimentalElevated plasma glypicans are associated with organ failure in patients with infection[Pubmed: 30618011]
VALUE OF SERUM GLYPICAN 3 AS A PREDICTIVE TOOL FOR EARLY DIAGNOSIS OF HEPATOCELLULAR CARCINOMA (HCC)[]
ADVANCED RESEARCHESSearch for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C[]
Life SciSelenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and …[Pubmed:35640776]