武汉云克隆科技股份有限公司

27kDa热休克蛋白(Hsp27)单克隆抗体

Monoclonal Antibody to Heat Shock Protein 27 (Hsp27)

HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

  • 27kDa热休克蛋白(Hsp27)单克隆抗体产品包装(模拟)
  • 27kDa热休克蛋白(Hsp27)单克隆抗体产品包装(模拟)
  • 27kDa热休克蛋白(Hsp27)单克隆抗体DAB staining on IHC-P; Sample: Human Brain Tissue; Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • 27kDa热休克蛋白(Hsp27)单克隆抗体DAB staining on IHC-P;
    Sample: Human Stomach Tissue;
    Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 27kDa热休克蛋白(Hsp27)单克隆抗体DAB staining on IHC-P;
    Sample: Human Kidney Tissue;
    Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 27kDa热休克蛋白(Hsp27)单克隆抗体DAB staining on IHC-P;
    Sample: Human Prostate Tissue;
    Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 27kDa热休克蛋白(Hsp27)单克隆抗体DAB staining on IHC-P;
    Sample: Human Liver Tissue;
    Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 27kDa热休克蛋白(Hsp27)单克隆抗体DAB staining on IHC-P;
    Sample: Human Lung cancer Tissue;
    Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 27kDa热休克蛋白(Hsp27)单克隆抗体DAB staining on IHC-P;
    Sample: Porcine Liver Tissue
    Primary Ab: 30µg/ml Mouse Anti-Human Hsp27 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAA693Hu25.jpgWestern Blot; Sample: Lane1: Human Lung lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: Porcine Heart lysate Primary Ab: 2µg/ml Mouse Anti-Human Hsp27 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • 27kDa热休克蛋白(Hsp27)单克隆抗体Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate
    Primary Ab: 0.2µg/ml Mouse Anti-Human Hsp27 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对Hsp27的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别Hsp27。

用法

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
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参考文献

杂志参考文献
Animal Science JournalThe effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells[PubMed: 26467738]
Nature CommunicationsHSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis[Pubmed:29650953]
Advances in Experimental Medicine and BiologyMolecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease[Pubmed:29429028]
Diabetes Research and Clinical PracticeThe beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity[Pubmed: 31102684]
Journal of Immunology ResearchDamage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients[]
Journal of Clinical MedicineDamage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort[Pubmed: 32210109]
MedicineRegulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive …[Pubmed:35687771]
Beilstein ArchivesImmunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation[]