白介素35(IL35)单克隆抗体
Monoclonal Antibody to Interleukin 35 (IL35)
- 编号MAC008Hu21
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 来源单抗制备
- 宿主小鼠
- 效价(克隆号)C1
- Ig亚型 IgG2b Kappa
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- 免疫原RPC008Hu01-白介素35(IL35)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度1mg/ml
- 且适物种-
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载英文说明书 中文说明书
- 规格20µl100µl200µl1ml10ml
- 价格¥ 666¥ 1554¥ 2220¥ 5550¥ 22200
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
Figure.Western Blot; Sample: Recombinant IL35, Human.
通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对IL35的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别IL35。
用法
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC008Hu01 | 白介素35(IL35)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC008Hu01 | 白介素35(IL35)活性蛋白 | Cell culture; Activity Assays. |
| PAC008Hu01 | 白介素35(IL35)多克隆抗体 | WB; IHC |
| MAC008Hu25 | 白介素35(IL35)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC008Hu22 | 白介素35(IL35)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC008Hu23 | 白介素35(IL35)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC008Hu24 | 白介素35(IL35)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC008Hu29 | 白介素35(IL35)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC008Hu21 | 白介素35(IL35)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC008Hu28 | 白介素35(IL35)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC008Hu26 | 白介素35(IL35)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC008Hu27 | 白介素35(IL35)单克隆抗体 | WB; IHC; ICC; IP. |
| LAC008Hu72 | 白介素35(IL35)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEC008Hu | 白介素35(IL35)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEC008Hu | 白介素35(IL35)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC008Hu | 白介素35(IL35)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSC008Hu01 | 白介素35(IL35)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSC008Hu01 | 白介素35(IL35)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Oncology Letters | Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia.[PubMed: 22783403] |
| Journal of Maternal-Fetal and Neonatal Medicine | Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease[Pubmed: 24175856] |
| Journal of Reproductive Immunology | What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?[Pubmed: 24368037] |
| Human Immunology | The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia[ScienceDirect: S0198885913005776] |
| Folia Biologica (Praha) | Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers[Pubmed:24785110] |
| Molecular Medicine Reports | Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis[Pubmed:25323532] |
| Rom J Morphol Embryol. | The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy[Pubmed:25611266] |
| Journal of Immunotherapy | Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity[PubMed: 25962106] |
| PLOS ONE | Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis[PubMed: 26204444] |
| Rheumatology (Oxford) | Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease[PubMed: 26231346] |
| Cytokine | Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia[PubMed: 26282938] |
| J Biol Chem | Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway[PubMed: 26085094] |
| Br J Haematol | Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia[PubMed: 25640666] |
| Autoimmunity Reviews | New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literature[Pubmed:26876383] |
| Clinical research | Increased plasma concentrations of interleukin 35 in patients with coronary artery disease[Journal:-19] |
| Clinical Rheumatology | The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis[pubmed:27502600] |
| Respiratory Medicine | Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis[pubmed:27492538] |
| International Journal of Molecular Medicine | Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines.[pubmed:27460435] |
| 上海口腔医学 | IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析[QK:98604X] |
| Reproductive Sciences | Upregulation of Interleukin 35 in Patients With Endometriosis Stimulates Cell Proliferation[pubmed:28659009] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | IL‑35 may maintain homeostasis of the immune microenvironment in periodontitis[issn:2542-3975] |
| The Journal of Rheumatology | Synoviocytes-derived Interleukin 35 Potentiates B Cell Response in Patients with Osteoarthritis and Rheumatoid Arthritis[pubmed:29247146] |
| Cellular & Molecular Immunology | Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitro[pubmed:28713164] |
| Molecular Medicine Reports | Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia.[pubmed:29257310] |
| Asian pacific journal of allergy and immunology | Evaluation of serum interleukin- 35 level in children with persistent asthma.[pubmed:27996283] |
| PLoS One | Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio[pubmed:29236782] |
| Hepatology Communications | Significant association between FOXP3 gene polymorphism and steroid-resistant acute rejection in living donor liver transplantation[10.1002/hep4.1052] |
| International Journal of Clinical and Experimental Medicine | Clinical significance of IL-35 expression in the progression of clear cell renal cell carcinoma [ISSN:1940-5901] |
| OncoTargets and Therapy | Interleukin-35 as a predictor of prostate cancer in patients undergoing initial prostate biopsy.[pubmed:28761357] |
| Annals of Rheumatic Diseases | AB0734 Serum il-35 levels in systemic sclerosis and relationship with clinical features[10.1136:annrheumdis-2018-eular.2605] |
| Tohoku Journal of Experimental Medicine | Interleukin-35 as a New Biomarker of Renal Involvement in Lupus Nephritis Patients[Pubmed:29576585] |
| Turkish Journal Of Medical Sciences | The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility[Pubmed:29914245] |
| Oncotarget | Pro-tumoral immune cell alterations in wild type and Shb-deficient mice in response to 4T1 breast carcinomas[Pubmed:29721156] |
| Journal of Clinical Rheumatology | Serum Interleukin 35 Levels in Systemic Sclerosis and Relationship With Clinical Features.[Pubmed: 30431486] |
| Oncology Letters | Interleukin‑35 is associated with the tumorigenesis and progression of prostate cancer[] |
| Journal of Clinical Investigation | Plasmacytoid dendritic cells protect against immune-mediated acute liver injury via IL-35[Pubmed: 31264967] |
| Cells | CD4+ FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired[Pubmed: 32111105] |
| Cytokine | Interleukin-35 in idiopathic inflammatory myopathies[Pubmed: 33128920] |
| FEBS Open Bio | IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1[Pubmed: 33064893] |
| Cancer Cell International | Interleukin-35 promotes progression of prostate cancer and inhibits anti-tumour immunity[Pubmed: 33041668] |
| Journal of Neuroinflammation | Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain[Pubmed: 32375811] |
| Int Immunopharmacol | Association of IL-35 expression and gene polymorphisms in rheumatoid arthritis[33307515] |
| Archives of Medical Science | Decreased interleukin-35 levels and CD4+ EBI3+ T cells in patients with type 1 diabetes and the effects of the antibody against CD20 (rituximab)[33488880] |
| Asia Pac Allergy | Anti-inflammatory roles of interleukin-35 in the pathogenesis of Japanese cedar pollinosis[34386410] |
| Int Immunopharmacol | Pentosan Polysulfate Sodium augments the therapeutic effect of 5-Aminosalicylic Acid in DSS colitis model; the role of IL-35 expression[Pubmed:35247859] |
| Biology and Medical Researches | Association of three single nucleotide polymorphisms of interleukin-35 for detection and prediction of women with breast cancer in Erbil city[] |
| Research Square | Analysis of IL-10 and IL-35 in DPP-4 inhibitor-related bullous pemphigoid[] |