穿孔素1(PRF1)重组蛋白
Recombinant Perforin 1 (PRF1)
FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein
- 编号RPB317Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位细胞膜, 分泌, 细胞质, 内体
- 预测分子量63.4kDa
- 实际分子量62kDa(差异分析请参阅说明书)
- 片段与标签Lys32~Phe316 with N-terminal His and GST Tag
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点7.0
- 应用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，请参见活性蛋白。 - 下载英文说明书 中文说明书
- 规格10µg50µg200µg1mg5mg
- 价格¥ 1440¥ 3600¥ 7200¥ 21600¥ 54000
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
Figure . Gene Sequencing (extract)
Figure. SDS-PAGE
通过ISO 9001、ISO 13485质量体系认证
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB317Hu02 | 穿孔素1(PRF1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB317Hu01 | 穿孔素1(PRF1)活性蛋白 | Cell culture; Activity Assays. |
| RPB317Hu01 | 穿孔素1(PRF1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB317Hu02 | 穿孔素1(PRF1)活性蛋白 | Cell culture; Activity Assays. |
| PAB317Hu01 | 穿孔素1(PRF1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB317Hu81 | 穿孔素1(PRF1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB317Hu71 | 穿孔素1(PRF1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB317Hu22 | 穿孔素1(PRF1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB317Hu21 | 穿孔素1(PRF1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB317Hu | 穿孔素1(PRF1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB317Hu | 穿孔素1(PRF1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Diagnostic Microbiology and Infectious Disease | Granzyme B as a diagnostic marker of tuberculosis in patients with and without HIV coinfection[Pubmed:26915636] |
| FASEB JOURNAL | The immunoregulatory effects of CD8 T-cell–derived perforin on diet-induced nonalcoholic steatohepatitis[Pubmed: 30951375] |
| Theranostics | A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumors[Pubmed: 31695790] |
| Mol Immunol | CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection[Pubmed: 33223223] |
| INVESTIGATIONAL NEW DRUGS | Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors[Pubmed: 32772342] |
| Am J Transl Res | Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma[34377237] |
| Nat Biomed Eng | Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy[34873307] |
| J Exp Med | Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion[Pubmed:35061012] |