武汉云克隆科技股份有限公司

CD73分子(CD73)多克隆抗体

Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

NT5E; NT5-E; E5NT; E5-NT; NTE; EN; ENT; 5'-Nucleotidase, Ecto

  • CD73分子(CD73)多克隆抗体产品包装(模拟)
  • CD73分子(CD73)多克隆抗体产品包装(模拟)
  • PAB250Bo01.jpgFigure. Western Blot; Sample: Recombinant NT5E, Bovine.
  • CD73分子(CD73)多克隆抗体Western Blot; Sample: Bovine Cerebrum lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Bovine NT5E Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CD73分子(CD73)多克隆抗体Knockout Varification:
    Lane 1: Wild-type K562 cell lysate;
    Lane 2: NT5E knockout K562 cell lysate;
    Predicted MW: 63kd
    Observed MW: 70kd
    Primary Ab: 3µg/ml Rabbit Anti-Bovine NT5E Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CD73分子(CD73)多克隆抗体Western Blot; Sample: Bovine Liver lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Bovine NT5E Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CD73分子(CD73)多克隆抗体Western Blot; Sample: Human K562 cell lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Bovine NT5E Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对CD73的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CD73。

用法

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

相关产品

编号适用物种：Bos taurus; Bovine (Cattle，牛)应用(仅供研究使用，不用于临床诊断！)
RPB250Bo01CD73分子(CD73)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB250Bo01CD73分子(CD73)多克隆抗体WB; IHC; ICC; IP.

参考文献

杂志参考文献
Journal of AutoimmunityThe expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation[Pubmed:25640206]
TalantaSimultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment …[10.1016:j.talanta.2018.01.044]
Cancer BiomarkersExpression and clinical significance of serum NT5E protein in patients with colorectal cancer[Pubmed: 30932882]