谷氨酸半胱氨酸连接酶催化亚基(GCLC)多克隆抗体
Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC)
GCS; GLCL; GLCLC; Gamma-glutamylcysteine synthetase; Gamma-ECS
- 编号PAD759Ra01
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原RPD759Ra01-谷氨酸半胱氨酸连接酶催化亚基(GCLC)重组蛋白
- 缓冲液成份磷酸盐缓冲液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状液体
- 浓度500µg/mL
- 且适物种Mus musculus (Mouse，小鼠)
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载英文说明书 中文说明书
- 规格20µl100µl200µl1ml10ml
- 价格¥ 684¥ 1596¥ 2280¥ 5700¥ 22800
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产品包装(模拟)
产品包装(模拟)
DAB staining on IHC-P; Samples: Rat Cerebrum Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat GCLC Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Intestine Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat GCLC Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Uterus Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat GCLC Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Testis Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat GCLC Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat GCLC Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat GCLC Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Testis Tissue
Primary Ab: 20µg/ml Rabbit Anti-Rat GCLC Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Rat GCLC Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
WB图
Western Blot; Sample: Rat Kidney lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat GCLC Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat GCLC Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对GCLC的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别GCLC。
用法
免疫印迹：0.2-2µg/mL;1:250-2500
免疫组织化学：5-20µg/mL;1:25-100
免疫细胞化学：5-20µg/mL;1:25-100
最佳稀释倍数最终由用户决定。
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
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相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPD759Ra01 | 谷氨酸半胱氨酸连接酶催化亚基(GCLC)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD759Ra01 | 谷氨酸半胱氨酸连接酶催化亚基(GCLC)多克隆抗体 | WB; IHC; ICC; IP. |
| MAD759Ra21 | 谷氨酸半胱氨酸连接酶催化亚基(GCLC)单克隆抗体 | WB; IHC; ICC; IP. |
| SED759Ra | 谷氨酸半胱氨酸连接酶催化亚基(GCLC)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD759Ra | 谷氨酸半胱氨酸连接酶催化亚基(GCLC)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Oxidative Medicine and Cellular Longevity | Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects[pubmed:28798861] |
| Journal of Functional Foods | Effects of maternal serine supplementation on high-fat diet-induced oxidative stress and epigenetic changes in promoters of glutathione synthesis-related genes in …[10.1016:j.jff.2018.05.067] |