武汉云克隆科技股份有限公司

60kD热休克蛋白(Hsp60)多克隆抗体

Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

  • 60kD热休克蛋白(Hsp60)多克隆抗体产品包装(模拟)
  • 60kD热休克蛋白(Hsp60)多克隆抗体产品包装(模拟)
  • 60kD热休克蛋白(Hsp60)多克隆抗体DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 60kD热休克蛋白(Hsp60)多克隆抗体DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 60kD热休克蛋白(Hsp60)多克隆抗体DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 60kD热休克蛋白(Hsp60)多克隆抗体DAB staining on IHC-P;
    Samples: Human Stomach cancer Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 60kD热休克蛋白(Hsp60)多克隆抗体DAB staining on IHC-P;
    Samples: Human Breast cancer Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 60kD热休克蛋白(Hsp60)多克隆抗体DAB staining on IHC-P;
    Samples: Human Prostate Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA822Hu02.jpgWestern Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Primary Ab: 0.01µg/ml Rabbit Anti-Human Hsp60 Antibody Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 60kD热休克蛋白(Hsp60)多克隆抗体Western Blot; Samples: Lane1: MCF7 cell lysate; Lane2: A549 cell lysate;
    Primary Ab: 0.01µg/ml Rabbit Anti-Human Hsp60 Antibody
    Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 60kD热休克蛋白(Hsp60)多克隆抗体HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human HSPD1 Polyclonal Antibody (Catalog # PAA822Hu02, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • 60kD热休克蛋白(Hsp60)多克隆抗体Human Hela cell was stained with 20µg/ml rabbit Anti-human Hsp60 Polyclonal Antibody (Catalog PAA822Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对Hsp60的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别Hsp60。

用法

Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

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编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
RPA822Hu0260kD热休克蛋白(Hsp60)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
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参考文献

杂志参考文献
Sci RepHsp60 response in experimental and human temporal lobe epilepsy[PubMed: 25801186]
Cellular and Molecular Gastroenterology and HepatologySenescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro[Pubmed: 30878663]
Breast Cancer Res TreatActivation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer[Pubmed: 32601970]