武汉云克隆科技股份有限公司

70kDa热休克蛋白5(HSPA5)多克隆抗体

Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)

MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78

  • 70kDa热休克蛋白5(HSPA5)多克隆抗体产品包装(模拟)
  • 70kDa热休克蛋白5(HSPA5)多克隆抗体产品包装(模拟)
  • 70kDa热休克蛋白5(HSPA5)多克隆抗体DAB staining on IHC-P;
    Sample: Mouse Cerebrum Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat HSPA5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 70kDa热休克蛋白5(HSPA5)多克隆抗体DAB staining on IHC-P;
    Sample: Mouse Cerebellum Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat HSPA5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 70kDa热休克蛋白5(HSPA5)多克隆抗体DAB staining on IHC-P;
    Sample: Mouse Testis Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat HSPA5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC343Ra01.jpgWestern Blot; Samples: Lane1: Mouse Liver lysate; Lane2: Mouse Testis lysate; Lane3: A549 cell lysate; Primary Ab: 0.1µg/ml Rabbit Anti-Rat HSPA5 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对HSPA5的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别HSPA5。

用法

Western blotting: 0.5-2µg/mL;1:130-520
Immunohistochemistry: 5-20µg/mL;1:13-52
Immunocytochemistry: 5-20µg/mL;1:13-52
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

相关产品

编号适用物种：Rattus norvegicus (Rat，大鼠)应用(仅供研究使用，不用于临床诊断！)
RPC343Ra0170kDa热休克蛋白5(HSPA5)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAC343Ra0170kDa热休克蛋白5(HSPA5)多克隆抗体WB,IHC
MAC343Ra2170kDa热休克蛋白5(HSPA5)单克隆抗体WB; IHC; ICC; IP.
SEC343Ra70kDa热休克蛋白5(HSPA5)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMC343Ra70kDa热休克蛋白5(HSPA5)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSC343Ra0170kDa热休克蛋白5(HSPA5)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
41Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes.[PubMed: 20522592]
pharmacological researchNeuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors[Pubmed: 31048034]
EPILEPSY & BEHAVIORFenfluramine acts as a positive modulator of sigma-1 receptors[Pubmed: 32169824]
Journal of OncologyUsing Serological Proteome Analysis to Identify and Evaluate Anti-GRP78 Autoantibody as Biomarker in the Detection of Gastric Cancer[33381181]
HeliyonGlucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior[]
Medicine (Baltimore)Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients[34414940]