武汉云克隆科技股份有限公司

M2-型丙酮酸激酶(PKM2)多克隆抗体

Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)

M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein; Pyruvate Kinase, Muscle

  • M2-型丙酮酸激酶(PKM2)多克隆抗体产品包装(模拟)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体产品包装(模拟)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体DAB staining on IHC-P;
    Samples: Human Colorectal cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体DAB staining on IHC-P;
    Samples: Human Breast cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体DAB staining on IHC-P;
    Samples: Human Pancreatic cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体DAB staining on IHC-P;
    Sample: Mouse Colon Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体DAB staining on IHC-P;
    Sample: Mouse Lung Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体DAB staining on IHC-P;
    Sample: Mouse Lung Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体FITC staining on IF;<br/>Samples: Human MCF7 cell; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human PKM2 Antibody<br/>Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb18)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体AF488 staining on IF;
    Sample: Hela cell
    Primary Ab: 20µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA588Hu02.jpgFigure. Western Blot; Sample: Recombinant PKM2, Human.
  • M2-型丙酮酸激酶(PKM2)多克隆抗体Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: K562 cell lysate; Lane4: MCF7 cell lysate
    Primary Ab: 0.4µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PKM2 Polyclonal Antibody (Catalog PAA588Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对PKM2的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别PKM2。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
RPA588Hu01M2-型丙酮酸激酶(PKM2)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA588Hu02M2-型丙酮酸激酶(PKM2)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA588Hu03M2-型丙酮酸激酶(PKM2)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA588Hu02M2-型丙酮酸激酶(PKM2)活性蛋白Cell culture; Activity Assays.
PAA588Hu02M2-型丙酮酸激酶(PKM2)多克隆抗体WB; IHC; IF;FCM
PAA588Hu03M2-型丙酮酸激酶(PKM2)多克隆抗体WB; IHC; ICC/IF
PAA588Hu01M2-型丙酮酸激酶(PKM2)多克隆抗体WB; IHC; ICC/IF; FCM
FAA588Hu01抗M2-型丙酮酸激酶(PKM2)多克隆抗体Flow cytometry.
FAA588Hu03抗M2-型丙酮酸激酶(PKM2)多克隆抗体Flow cytometry.
MAA588Hu24M2-型丙酮酸激酶(PKM2)单克隆抗体WB
MAA588Hu22M2-型丙酮酸激酶(PKM2)单克隆抗体WB
MAA588Hu23M2-型丙酮酸激酶(PKM2)单克隆抗体WB; ICC/IF
MAA588Hu21M2-型丙酮酸激酶(PKM2)单克隆抗体WB
SEA588HuM2-型丙酮酸激酶(PKM2)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA588HuM2-型丙酮酸激酶(PKM2)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

参考文献

杂志参考文献
Cancer ResearchAntigen Presentation by Dendritic Cells in Tumors Is Disrupted by Altered Metabolism that Involves Pyruvate Kinase M2 and Its Interaction with SOCS3[AACR: 70189]
Molecular BioSystemsAbnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer[Pubmed:25483567]
Tumour BiolM2 isoform of pyruvate kinase (PKM2) is upregulated in Kazakh’s ESCC and promotes proliferation and migration of ESCC cells[PubMed: 26404132]
OncotargetIdentification of a serum biomarker panel for the differential diagnosis of cholangiocarcinoma and primary sclerosing cholangitis[Pubmed:29707118]
Journal of NeurotraumaActeoside improves muscle atrophy and motor function by inducing new myokine secretion in chronic spinal cord injury[Pubmed: 30318996]
Cell ReportsSecreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway[Pubmed: 32049010]
Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC)[]
Cancer Cell InternationalBufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins[]
Cancer Cell IntBufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression[34376212]
Life (Basel)Putative Association between Low Baseline Gene Expression in the Peripheral Blood and Clinical Remission in Rheumatoid Arthritis Patients Treated with Tofacitinib[34947916]