CD146分子(CD146)重组抗体
Recombinant Antibody to Cluster Of Differentiation 146 (CD146)
MCAM; MUC18; Melanoma Cell Adhesion Molecule; Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; S-endo 1 endothelial-associated antigen
- 编号RAB670Hu21
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 来源重组单抗制备
- 宿主小鼠
- 效价(克隆号)-
- Ig亚型 IgG
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- 免疫原-
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度1mg/mL
- 且适物种-
- 应用WB; IF; ICC; IHC; IP; FCM.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载英文说明书 中文说明书
- 规格20µl100µl200µl1ml10ml
- 价格¥ 1080¥ 2520¥ 3600¥ 9000¥ 36000
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
Human Hela cell line was stained with 5-10µg/ml mouse Anti-human CD146 Recombinant Antibodies (Catalog # RAB670Hu21, red histogram) or Isotype control antibody (Catalog # IS067-Mu02, green histogram), followed by 1µg/ml PE-conjugated Anti-mouse IgG Secondary Antibody (Catalog # SAA544Mu14).
Human peripheral blood lymphocytes were stained with 1µg/ml mouse Anti-human CD146 Recombinant Antibody (Catalog RAB670Hu21, green histogram) or Isotype control antibody (Catalog IS067-Mu03, blue histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18)
通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对CD146的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CD146。
用法
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:10μg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APB670Hu01 | CD146分子(CD146)活性蛋白 | Cell culture; Activity Assays. |
| RPB670Hu01 | CD146分子(CD146)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB670Hu01 | CD146分子(CD146)多克隆抗体 | WB; ICC/IF |
| FAB670Hu01 | 抗CD146分子(CD146)多克隆抗体 | FCM |
| FAB670Hu81 | 抗CD146分子(CD146)多克隆抗体(异硫氰酸荧光素标记) | FCM |
| RAB670Hu21 | CD146分子(CD146)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAB670Hu22 | CD146分子(CD146)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB670Hu | CD146分子(CD146)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB670Hu | CD146分子(CD146)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Clinical Rheumatology | Evaluation of various endothelial biomarkers in ankylosing spondylitis[PubMed: 21556780] |
| Inflammation | The Effect of Regular Colchicine Treatment on Biomarkers Related with Vascular Injury in Newly Diagnosed Patients with Familial Mediterranean Fever[SpringerLink: m7128503g576w808] |
| Oncotarget | Selective targeting of liver cancer with the endothelial marker CD146[Pubmed:25238265] |
| Future Medicine | Melanoma cell adhesion molecule as an emerging biomarker with prognostic significance in systolic heart failure[pubmed:27355366] |