武汉云克隆科技股份有限公司

绿色荧光蛋白(GFP)重组抗体

Recombinant Antibody to Green Fluorescent Protein (GFP)

    • 绿色荧光蛋白(GFP)重组抗体产品包装(模拟)
    • 绿色荧光蛋白(GFP)重组抗体产品包装(模拟)
    • 绿色荧光蛋白(GFP)重组抗体Briefly, biotin labelled GFP Nano-antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to GFP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed 3 times with PBST and incubated for 0.5 h at 37℃ with SA labelled HRP. After incubation with SA labelled HRP, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of GFP and GFP Nano-antibody was shown in Figure 1, and the ED50 for this effect is 0.0187 ug/ml.
    • 绿色荧光蛋白(GFP)重组抗体GFP was immunoprecipitated 293F whole cell extract (Expressing GFP) with 2 µg of RAD025Ge71 and 20 µl of Nickel pillar magnetic beads ( ). 
      Western blot was perfomed on the immunoprecipitate using 0.05µg/ml MAD025Ge21 and 0.2µg/mL SAA544Mu19.
      Lane1 (input): 293F whole cell lysate.
      Lane2 ( ): RAD025Ge71 in 293F whole cell lysate.
      Lane3 (-): No antibody was added to the 293F whole cell lysate.
    • Certificate通过ISO 9001、ISO 13485质量体系认证

    特异性

    该抗体是针对GFP的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别GFP。

    用法

    Western blotting: 0.5-2µg/mL;
    Immunohistochemistry: 5-20µg/mL;
    Immunocytochemistry: 5-20µg/mL;
    Optimal working dilutions must be determined by end user.

    储存

    经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

    稳定性

    热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

    赠品

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    编号适用物种：Pan-species (General，通用)应用(仅供研究使用，不用于临床诊断！)
    EPD025Ge51绿色荧光蛋白(GFP)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
    RPD025Ge01绿色荧光蛋白(GFP)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
    RPD025Ge02绿色荧光蛋白(GFP)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
    PAD025Ge07绿色荧光蛋白(GFP)多克隆抗体WB; IHC; ICC; IP.
    PAD025Ge02绿色荧光蛋白(GFP)多克隆抗体WB; IHC; ICC; IP.
    PAD025Ge51绿色荧光蛋白(GFP)多克隆抗体WB; IHC; ICC; IP.
    MAD025Ge21绿色荧光蛋白(GFP)单克隆抗体WB; IHC; ICC; IP.
    MAD025Ge22绿色荧光蛋白(GFP)单克隆抗体WB; IHC; ICC; IP.
    RAD025Ge71绿色荧光蛋白(GFP)重组抗体WB; IF; ICC; IHC; IP; FCM.
    MAD025Ge51绿色荧光蛋白(GFP)单克隆抗体WB; IHC; ICC; IP.
    SED025Ge绿色荧光蛋白(GFP)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
    AED025Ge抗绿色荧光蛋白抗体(Anti-GFP)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antibody Detection.
    LMD025Ge绿色荧光蛋白(GFP)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
    KSD025Ge01绿色荧光蛋白(GFP)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

    参考文献

    杂志参考文献
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    Microbiol SpectrJapanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells[Pubmed:35604158]