信号素4D(SEMA4D)重组蛋白
Recombinant Semaphorin 4D (SEMA4D)
CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D
- 编号RPB430Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位细胞膜
- 预测分子量16.2kDa
- 实际分子量18kDa(差异分析请参阅说明书)
- 片段与标签Phe502~Phe636 with N-terminal His Tag
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 97%
- 等电点8.8
- 应用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，请参见活性蛋白。 - 下载英文说明书 中文说明书
- 规格10µg50µg200µg1mg5mg
- 价格¥ 984¥ 2460¥ 4920¥ 14760¥ 36900
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB430Hu01 | 信号素4D(SEMA4D)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB430Hu01 | 信号素4D(SEMA4D)多克隆抗体 | WB; IHC; ICC; IP. |
| MAB430Hu22 | 信号素4D(SEMA4D)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB430Hu | 信号素4D(SEMA4D)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB430Hu | 信号素4D(SEMA4D)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Expert Opin Ther Targets. | Circulating semaphorin-4D and plexin-B1 levels in postmenopausal women with low bone mass: the 3-month effect of zoledronic acid, denosumab or teriparatide treatment[Pubmed:25395071] |
| Obesity (Silver Spring). | Genome-wide analysis identifies an african-specific variant in SEMA4D associated with body mass index.[pubmed:28296344] |
| Clinical Lymphoma, Myeloma & Leukemia | A Revised Staging System for Waldenström's Macroglobulinemia[10.1016/j.clml.2017.07.146] |
| Clinical Lymphoma, Myeloma & Leukemia | Extramedullary Multiple Myeloma: Clinical Features and Prognosis in Argentina[10.1016/j.clml.2017.07.144] |
| Blood Cancer Journal | Semaphorin 4D correlates with increased bone resorption, hypercalcemia, and disease stage in newly diagnosed patients with multiple myeloma[Pubmed:29748532] |
| Analytical Biochemistry | A high-sensitivity enzyme immunoassay for the quantification of soluble human semaphorin 4D in plasma[Pubmed: 30879960] |
| Journal of Allergy and Clinical Immunology-In Practice | Pathological and therapeutic implications of eosinophil-derived semaphorin 4D in eosinophilic chronic rhinosinusitis[Pubmed: 32035658] |