糖化血红蛋白A1c(HbA1c)检测试剂盒(酶联免疫吸附试验法,宽范围)
Wide-range ELISA Kit for Glycated Hemoglobin A1c (HbA1c)
Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc
特异性
本试剂盒用于检测糖化血红蛋白A1c(HbA1c)检测试剂盒(酶联免疫吸附试验法,宽范围)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的糖化血红蛋白A1c(HbA1c)检测试剂盒(酶联免疫吸附试验法,宽范围)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| EDTA plasma(n=5) | 89-96 | 93 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的糖化血红蛋白A1c(HbA1c)检测试剂盒(酶联免疫吸附试验法,宽范围)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中糖化血红蛋白A1c(HbA1c)检测试剂盒(酶联免疫吸附试验法,宽范围)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| EDTA plasma(n=5) | 84-91% | 90-104% | 80-93% | 95-103% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将糖化血红蛋白A1c(HbA1c)检测试剂盒(酶联免疫吸附试验法,宽范围)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| NPA190Mu01 | 糖化血红蛋白A1c(HbA1c)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA190Mu01 | 糖化血红蛋白A1c(HbA1c)多克隆抗体 | WB; IHC; ICC; IP. |
| WEA190Mu | 糖化血红蛋白A1c(HbA1c)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA190Mu | 糖化血红蛋白A1c(HbA1c)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA190Mu | 糖化血红蛋白A1c(HbA1c)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA190Mu | 糖化血红蛋白A1c(HbA1c)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA190Mu11 | 糖化血红蛋白A1c(HbA1c)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| J Am Coll Nutr | The Effect of Vitamin D Supplementation on Glycemic Control and Lipid Profile in Patients with Type 2 Diabetes Mellitus[PubMed: 26391639] |
| Journal of the American College of Nutrition | The Effect of Vitamin D Supplementation on Glycemic Control and Lipid Profile in Patients with Type 2 Diabetes Mellitus.[pubmed:26391639] |
| Journal of Endocrinology and Metabolism | Circulating Betatrophin and Hepatocyte Growth Factor in Type 2 Diabetic Patients: Their Relationship With Disease Prognosis[405] |
| Molecular nutrition & food research | Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro[pubmed:28105758] |
| 33 | d-Ribose as a Contributor to Glycated Haemoglobin[pubmed:29033370] |
| Journal of Functional Foods | Hypoglycemic effect of Hypericum attenuatum Choisy extracts on type 2 diabetes by regulating glucolipid metabolism and modulating gut microbiota[] |
| West Indian Medical Journal | Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.[] |
| Journal of Occupational and Environmental Hygiene | Stressors, allostatic load, and health outcomes among women hotel housekeepers: A pilot study[Pubmed: 30615593] |
| Cell Reports | Parabacteroides distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile Acids[Pubmed: 30605678] |
| Journal of Agricultural and Food Chemistry | Gymnemic acid alleviates type 2 diabetes mellitus and suppresses endoplasmic reticulum stress in vivo and in vitro[] |
| Journal of Clinical Medicine | 1H Nuclear Magnetic Resonance (NMR)-Based Cerebrospinal Fluid and Plasma Metabolomic Analysis in Type 2 Diabetic Patients and Risk Prediction for Diabetic …[Pubmed: 31248127] |
| Food & Function | Gymnemic Acid Alleviates Inflammation and Insulin Resistance via PPARδ-and NFκB-mediated pathways in db/db mice.[] |
| Obesity Surgery | Duodenal-Jejunal Bypass Ameliorates Type 2 Diabetes Mellitus by Activating Insulin Signaling and Improving Glucose Utilization in the Brain[Pubmed: 31605365] |
| Acta Diabetologica | Modulation of gut microbiota contributes to effects of intensive insulin therapy on intestinal morphological alteration in high-fat-diet-treated mice[Pubmed: 31749050] |
| Open Access Macedonian Journal of Medical Sciences | Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy[Pubmed: 32165929] |
| Cell Reports | Activation of a Specific Gut Bacteroides-Folate-Liver Axis Benefits for the Alleviation of Nonalcoholic Hepatic Steatosis[Pubmed: 32783933] |
| The effects of different intensity endurance and resistance exercise on diabetic-related blood profiles in impaired glucose tolerance mice[] | |
| Revista Internacional de Andrologia | Relation of pharmacopenile duplex ultrasonography parameters and glycated hemoglobin (HbA1c) in diabetic patients with erectile dysfunction[33334711] |
| Advanced Theory and Simulations? | Identification of Key Contributive Compounds in a Herbal Medicine: A Novel Mathematic¡ªBiological Evaluation Approach[] |
| Dis Markers | The Roles of Liver Inflammation and the Insulin Signaling Pathway in PM2. 5 Instillation-Induced Insulin Resistance in Wistar Rats[34745386] |
| Antioxidants | Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice[Pubmed:35740008] |
| P001–Inv. Clínica[] |
