可罗索(KL)检测试剂盒(酶联免疫吸附试验法,宽范围)
Wide-range ELISA Kit for Klotho (KL)
KLa; Klotho Alpha
特异性
本试剂盒用于检测可罗索(KL)检测试剂盒(酶联免疫吸附试验法,宽范围)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的可罗索(KL)检测试剂盒(酶联免疫吸附试验法,宽范围)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 87-105 | 96 |
| EDTA plasma(n=5) | 94-101 | 98 |
| heparin plasma(n=5) | 79-93 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的可罗索(KL)检测试剂盒(酶联免疫吸附试验法,宽范围)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中可罗索(KL)检测试剂盒(酶联免疫吸附试验法,宽范围)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 97-105% | 90-102% | 80-97% |
| EDTA plasma(n=5) | 85-92% | 93-101% | 99-105% | 99-105% |
| heparin plasma(n=5) | 80-99% | 97-104% | 88-103% | 80-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将可罗索(KL)检测试剂盒(酶联免疫吸附试验法,宽范围)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的可罗索(KL)检测试剂盒(酶联免疫吸附试验法,宽范围)与连接于固相载体上的抗体结合，然后加入生物素化的可罗索(KL)检测试剂盒(酶联免疫吸附试验法,宽范围)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的可罗索(KL)检测试剂盒(酶联免疫吸附试验法,宽范围)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPH757Mu03 | 可罗索(KL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPH757Mu02 | 可罗索(KL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPH757Mu01 | 可罗索(KL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH757Mu02 | 可罗索(KL)多克隆抗体 | WB; IHC; ICC; IP. |
| PAH757Mu01 | 可罗索(KL)多克隆抗体 | WB; IHC; ICC; IP. |
| LAH757Mu71 | 可罗索(KL)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAH757Mu81 | 可罗索(KL)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| SEH757Mu | 可罗索(KL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEH757Mu | 可罗索(KL)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCH757Mu | 可罗索(KL)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMH757Mu | 可罗索(KL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSH757Mu01 | 可罗索(KL)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
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| Mol Cell Endocrinol | The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism[PubMed: 26419931] |
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| Am J Nephrol | Klotho Deficiency Aggravates Tacrolimus-Induced Renal Injury via the Phosphatidylinositol 3-Kinase-Akt-Forkhead Box Protein O Pathway[Pubmed:27174564] |
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| Cellular Physiology and Biochemistry | Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury[Pubmed:29550818] |
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| Nephrology Dialysis Transplantation | High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease[Pubmed: 30189026] |
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| Expert Opinion on Therapeutic Targets | The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic rats[Pubmed: 30801205] |
| Mol Immunol | CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection[Pubmed: 33223223] |
| Journal of Clinical Hypertension | Effects of salt intervention on serum levels of Klotho influenced by salt sensitivity[Pubmed: 33164306] |
| CURRENT EYE RESEARCH | Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic rats[Pubmed: 32744464] |
| international immunopharmacology | Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats[Pubmed: 32438076] |
| Purinergic Signalling | P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy.[Pubmed: 32377919] |
| Oxidative Medicine and Cellular Longevity | Ameliorating Effect of Klotho Protein on Rat Heart during I/R Injury[Pubmed: 33123314] |
| NEUROSCIENCE | Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With Schizophrenia[Pubmed: 32612508] |
| Pharmacological Reports | ¦ÁKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy[33675964] |
| J Mol Neurosci | Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma Cells[Pubmed:35112226] |
| Reproductive Biology and Endocrinology | Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance[] |
| Journal of Ovarian Research | Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome[] |
