文献 Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity 发表在 Nature Medicine 原文链接

Abstract:During obesity, macrophage accumulation in adipose tissue propagates the chronic inflammation and insulin resistance associated with type 2 diabetes. The factors, however, that regulate the accrual of macrophages in adipose tissue are not well understood. Here we show that the neuroimmune guidance cue netrin-1 is highly expressed in obese but not lean adipose tissue of humans and mice, where it directs the retention of macrophages. Netrin-1, whose expression is induced in macrophages by the saturated fatty acid palmitate, acts via its receptor Unc5b to block their migration. In a mouse model of diet-induced obesity, we show that adipose tissue macrophages exhibit reduced migratory capacity, which can be restored by blocking netrin-1. Furthermore, hematopoietic deletion of Ntn1 facilitates adipose tissue macrophage emigration, reduces inflammation and improves insulin sensitivity. Collectively, these findings identify netrin-1 as a macrophage retention signal in adipose tissue during obesity that promotes chronic inflammation and insulin resistance.


使用试剂原文信息:ELISA assays. Insulin (90080; Crystal Chem), adiponectin (ab108785;Abcam), FFA (SFA­1; Zen Bio), TNF-a (88­7064­76; eBioscience) and netrin­1(E91827Mu; USCN Life science) levels were measured in the serum or cell supernatants using mouse standards according to manufacturer’s guidelines.

文献相关产品 SEB827HuSEB827Mu 由云克隆研发生产