双调蛋白(AREG)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Amphiregulin (AREG)
AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor
- 编号KSA006Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围15.6-1,000pg/mL
- 可适灵敏度6.5pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6048¥ 10080¥ 18144¥ 35280¥ 60480
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对双调蛋白(AREG)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA006Hu01 | 双调蛋白(AREG)活性蛋白 | Cell culture; Activity Assays. |
| RPA006Hu01 | 双调蛋白(AREG)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA006Hu01 | 双调蛋白(AREG)多克隆抗体 | WB; IHC |
| MAA006Hu23 | 双调蛋白(AREG)单克隆抗体 | IHC; ICC/IF |
| MAA006Hu25 | 双调蛋白(AREG)单克隆抗体 | IHC |
| MAA006Hu22 | 双调蛋白(AREG)单克隆抗体 | WB; IHC |
| MAA006Hu24 | 双调蛋白(AREG)单克隆抗体 | WB; IHC; ICC/IF |
| MAA006Hu26 | 双调蛋白(AREG)单克隆抗体 | WB; IHC |
| MAA006Hu21 | 双调蛋白(AREG)单克隆抗体 | IHC |
| MAA006Hu27 | 双调蛋白(AREG)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA006Hu29 | 双调蛋白(AREG)单克隆抗体 | WB; IHC; ICC; IP. |
| LAA006Hu76 | 双调蛋白(AREG)单克隆抗体(生物素标记) | IHC |
| SEA006Hu | 双调蛋白(AREG)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA006Hu | 双调蛋白(AREG)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA006Hu | 双调蛋白(AREG)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA006Hu01 | 双调蛋白(AREG)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA006Hu01 | 双调蛋白(AREG)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Onkologie | Amphiregulin and Epiregulin Expression in Colorectal Carcinoma and the Correlation with Clinicopathological Characteristics[Karger: 315380] |
| Br J Cancer. | Serum levels of hepatocyte growth factor and epiregulin are associated with the prognosis on anti-EGFR antibody treatment in KRAS wild-type metastatic colorectal cancer.[Pubmed:24800946] |
| Cancer Sci | Association between serum ligands and the skin toxicity of anti-epidermal growth factor receptor antibody in metastatic colorectal cancer[PubMed: 25707609] |
| J Cancer Res Clin Oncol | Amphiregulin confers trastuzumab resistance via AKT and ERK activation in HER2-positive breast cancer[PubMed: 26195282] |
| Oncotarget. | Serum level of hepatocyte growth factor is a novel marker of predicting theoutcome and resistance to the treatment with trastuzumab in HER2-positive patients with metastatic gastric cancer[Pubmed:26716644] |
| Acta Pharmacologica Sinica | Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi')[Pubmed: 19363516] |
| International Journal of Biological Sciences | CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17[] |
| Neoplasia | Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine[Pubmed: 33212364] |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets[Pubmed: 33164313] |