补体成分3a(C3a)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Complement Component 3a (C3a)

  • 补体成分3a(C3a)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 补体成分3a(C3a)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对补体成分3a(C3a)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志参考文献
22Immobilization of soluble complement receptor 1 on islets.[ScienceDirect: S0142961211002596]
Journal of Reproductive ImmunologyElevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia[PubMed: 23415845]
Journal of Antimicrobial ChemotherapyEfficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model[Pubmed: 23645587]
SpringerPlusTherapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats[Pubmed: 24353977]
Gynecol Obstet InvestComplement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia?[PubMed: 26485247]
Am J Reprod ImmunolHuman IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study[PubMed: 26388133]
Journal of NeuroimmunologyAcute and prolonged complement activation in the central nervous system during herpes simplex encephalitis[Pubmed:27235358]
Plos OneAlternative Pathway Inhibition by Exogenous Factor H Fails to Attenuate Inflammation and Vascular Leakage in Experimental Pneumococcal Sepsis in Mice[Pubmed:26872035]
Digital RepositoryDosagem de frações ativadas do sistema complemento em empiema induzido em ratos[10183]
Colloids Surf B Biointerfaces.Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns.[pubmed:28131092]
Pediatric PulmonologyAccuracy of complement activation product levels to detect infected pleural effusion in rats[pubmed:28474458]
Journal of ImmunologyThe Complement C3a–C3aR Axis Promotes Development of Thoracic Aortic Dissection via Regulation of MMP2 Expression[Pubmed:29367209]
Artificial Cells Nanomedicine and Biotechnology JournalLong term safety and immunological effects of a nanobiotherapeutic, bovine poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], after four weekly …[Pubmed:29873524]
CytotherapyMesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation[Doi: 10.1016/j.jcyt.2018.07.007]
Journal of NeuroVirologyIntrathecal complement activation by the classical pathway in tick-borne encephalitis[Pubmed: 30850976]
Clinical ImmunologyPathogenic roles of anti-C1q antibodies in recurrent pregnancy loss[]
Materials Science and Engineering: CHemocompatibility of biodegradable Zn-0.8 wt%(Cu, Mn, Li) alloys[]
NanomedicineComplement-mediated hypersensitivity reactions to an amphotericin B-containing lipid complex (Abelcet) in pediatric patients and anesthetized rats: Benefits of slow infusion[33549818]
EXPERIMENTAL EYE RESEARCHIncreased ratios of complement factors C3a to C3 in aqueous humor and serum mark glaucoma progression[33493474]
FASEB JC‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease[Pubmed:35503088]