武汉云克隆科技股份有限公司

亲环素A(CYPA)多克隆抗体

Polyclonal Antibody to Cyclophilin A (CYPA)

PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein

  • 亲环素A(CYPA)多克隆抗体产品包装(模拟)
  • 亲环素A(CYPA)多克隆抗体产品包装(模拟)
  • 亲环素A(CYPA)多克隆抗体DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CYPA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 亲环素A(CYPA)多克隆抗体DAB staining on IHC-P;
    Samples: Human Cerebrum Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 亲环素A(CYPA)多克隆抗体DAB staining on IHC-P;
    Sample: Porcine Kidney Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human CYPA Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 亲环素A(CYPA)多克隆抗体DAB staining on IHC-P;
    Sample: Porcine Testis Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human CYPA Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 亲环素A(CYPA)多克隆抗体AF488 staining on IF;
    Sample: Hela cell
    Primary Ab: 20µg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA979Hu01.jpgFigure. Western Blot; Sample: Recombinant CYPA, Human.
  • 亲环素A(CYPA)多克隆抗体Western Blot; Sample: Human Liver lysate;
    Primary Ab: 4µg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 亲环素A(CYPA)多克隆抗体Western Blot; Sample: Human Serum;
    Primary Ab: 4µg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 亲环素A(CYPA)多克隆抗体Western Blot; Sample: Rat Serum;
    Primary Ab: 4µg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 亲环素A(CYPA)多克隆抗体Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Mouse Cerebrum lysate; Lane3: HepG2 cell lysate; Lane4: Jurkat cell lysate
    Primary Ab: 0.05µg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 亲环素A(CYPA)多克隆抗体Western Blot; Samples: Lane1: Mouse Testis lysate; Lane2: Mouse Cerebrum lysate; Lane3: Rat Lung lysate; Lane4: Hela cell lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 亲环素A(CYPA)多克隆抗体Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CYPA Polyclonal Antibody (Catalog PAA979Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对CYPA的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CYPA。

用法

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Flow cytometry: 20μg/mL;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
RPA979Hu01亲环素A(CYPA)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
CPA979Hu21亲环素A(CYPA)卵白蛋白偶联物Immunogen; SDS-PAGE; WB.
PAA979Hu08亲环素A(CYPA)多克隆抗体WB; IHC; ICC; IP.
PAA979Hu01亲环素A(CYPA)多克隆抗体WB; IHC; ICC/IF; FCM
LAA979Hu81亲环素A(CYPA)多克隆抗体(异硫氰酸荧光素标记)WB; IHC; ICC; IF.
LAA979Hu71亲环素A(CYPA)多克隆抗体(生物素标记)WB; IHC; ICC.
MAA979Hu22亲环素A(CYPA)单克隆抗体WB; IHC; ICC/IF
MAA979Hu23亲环素A(CYPA)单克隆抗体WB
MAA979Hu24亲环素A(CYPA)单克隆抗体WB; IHC
MAA979Hu25亲环素A(CYPA)单克隆抗体WB
MAA979Hu21亲环素A(CYPA)单克隆抗体WB; IHC
FAA979Hu02抗亲环素A(CYPA)单克隆抗体FCM
FAA979Hu06抗亲环素A(CYPA)单克隆抗体FCM
FAA979Hu08抗亲环素A(CYPA)单克隆抗体FCM
LAA979Hu72亲环素A(CYPA)单克隆抗体(生物素标记)WB
SEA979Hu亲环素A(CYPA)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA979Hu亲环素A(CYPA)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
PSA979Hu01亲环素A(CYPA)抗体对ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA979Hu01亲环素A(CYPA)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
AtherosclerosisEMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation[PubMed: 19758589]
BiomarkersCyclophilin-A: a novel biomarker for untreated male essential hypertension[Pubmed: 24164594]
Cardiovascular DiabetologyPlasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease[Pubmed:24502618]
International Journal of Biological SciencesDecrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial Infarction[Pubmed:25552928]
MedicineCyclophilin A in Ruptured Intracranial Aneurysm[PubMed: 26426668]
Mol Cell BiochemPPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery disease[PubMed: 26702934]
Medicine (Baltimore)Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes Mellitus[PubMed: 26496315]
International Journal of CardiologyCyclophilin A modulates bone marrow-derived CD117(+) cells and enhances ischemia-induced angiogenesisvia the SDF-1/CXCR4 axis.[Pubmed:27057951]
Clinica Chimica ActaNovel findings of secreted cyclophilin A in diabetic nephropathy and its association with renal protection of dipeptidyl peptidase 4 inhibitor[pubmed:27823952]
Cardiovascular DiabetologyCyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus.[pubmed:27809851]
Clinical ScienceMetformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity[Pubmed:29382697]
Journal of Molecular HistologyCyclophilin a increases CD68 cell infiltration in rat experimental periodontitis[Pubmed:29318411]
Free Radical ResearchCyclophilin A and CD147 associate with progression of diabetic nephropathy[Pubmed: 30572748]
The Kaohsiung Journal of Medical SciencesAssociation of cyclophilin A level and pulse pressure in predicting recurrence of cerebral infarction[Pubmed: 31670477]
Cells.Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome Patients[Pubmed: 31936351]
JAMA NeurologyAPPENDIX A: Relationship between cyclophilin A levels and matrix metalloproteinase-9 activity in cerebrospinal fluid of cognitively normal apolipoprotein E4 carriers …[Pubmed: 24030206]
Menoufia Medical JournalUrinary podocalyxin and cyclophilin A: markers for early detection of type 2 diabetic nephropathy[]
ToxinsEvaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy[34437430]
Cell Death & DiseaseProteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death[Pubmed:35732625]