抗增殖细胞核抗原(PCNA)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Proliferating Cell Nuclear Antigen (PCNA)
Cyclin
- 编号KSA591Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.059ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7416¥ 12360¥ 22248¥ 43260¥ 74160
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
试剂盒中的抗体对抗增殖细胞核抗原(PCNA)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA591Hu01 | 抗增殖细胞核抗原(PCNA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAA591Hu01 | 抗抗增殖细胞核抗原(PCNA)多克隆抗体 | Loading Control of WB. |
| PAA591Hu01 | 抗增殖细胞核抗原(PCNA)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA591Hu21 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA591Hu22 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA591Hu23 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA591Hu25 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA591Hu24 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC; ICC; IP. |
| CAA591Hu22 | 抗抗增殖细胞核抗原(PCNA)单克隆抗体 | Loading Control of WB. |
| FAA591Hu02 | 抗抗增殖细胞核抗原(PCNA)单克隆抗体 | FCM |
| FAA591Hu04 | 抗抗增殖细胞核抗原(PCNA)单克隆抗体 | FCM |
| KSA591Hu01 | 抗增殖细胞核抗原(PCNA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
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