武汉云克隆科技股份有限公司

抗增殖细胞核抗原(PCNA)单克隆抗体

Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)

Cyclin

  • 抗增殖细胞核抗原(PCNA)单克隆抗体产品包装(模拟)
  • 抗增殖细胞核抗原(PCNA)单克隆抗体产品包装(模拟)
  • 抗增殖细胞核抗原(PCNA)单克隆抗体DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 20µg/ml Mouse Anti-Human PCNA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • 抗增殖细胞核抗原(PCNA)单克隆抗体DAB staining on IHC-P;
    Sample: Human Breast cancer Tissue
    Primary Ab: 20µg/ml Mouse Anti-Human PCNA Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗增殖细胞核抗原(PCNA)单克隆抗体FITC staining on IF;
    Sample: MCF7 cell
    Primary Ab: 20µg/ml Mouse Anti-Human PCNA Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • MAA591Hu21.jpgFigure. Western Blot; Sample: Recombinant PCNA.
  • 抗增殖细胞核抗原(PCNA)单克隆抗体Western Blot Sample: Lane1: Porcine Kidney lysate; Lane2: Bovine Testis lysate; Lane3: Caprine Lung lysate; Lane4: Caprine Testis lysate; Lane5: Cavia Lung lysate; Lane6: Cavia Colon lysate; Lane7: Cavia Thymus lysate; Lane8: Equine Liver lysate
    Primary Ab: 1μg/ml Mouse Anti-Human PCNA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对PCNA的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别PCNA。

用法

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
RPA591Hu01抗增殖细胞核抗原(PCNA)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
CAA591Hu01抗抗增殖细胞核抗原(PCNA)多克隆抗体Loading Control of WB.
PAA591Hu01抗增殖细胞核抗原(PCNA)多克隆抗体WB; IHC; ICC; IP.
MAA591Hu21抗增殖细胞核抗原(PCNA)单克隆抗体WB; IHC; ICC; IP.
MAA591Hu22抗增殖细胞核抗原(PCNA)单克隆抗体WB; IHC; ICC; IP.
MAA591Hu23抗增殖细胞核抗原(PCNA)单克隆抗体WB; IHC; ICC; IP.
MAA591Hu25抗增殖细胞核抗原(PCNA)单克隆抗体WB; IHC; ICC; IP.
MAA591Hu24抗增殖细胞核抗原(PCNA)单克隆抗体WB; IHC; ICC; IP.
CAA591Hu22抗抗增殖细胞核抗原(PCNA)单克隆抗体Loading Control of WB.
FAA591Hu02抗抗增殖细胞核抗原(PCNA)单克隆抗体FCM
FAA591Hu04抗抗增殖细胞核抗原(PCNA)单克隆抗体FCM
KSA591Hu01抗增殖细胞核抗原(PCNA)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
Tissue and CellHuman umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects[Pubmed:27233913]
Food and Chemical ToxicologyAntioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats[Pubmed:30026088]
Life SciencesMalaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats[Pubmed: 30205096]
Archives of Physiology and BiochemistryDiabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin[Pubmed: 30513216]
European journal of medicinal chemistrySynthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity[Pubmed: 31216504]
Antioxidants (Basel).The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets[Pubmed: 31426309]
AntioxidantsOxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian …[Pubmed: 31600920]
TOXICOLOGYTert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis[Pubmed: 32565124]
BIOMEDICINE & PHARMACOTHERAPYProtective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats[Pubmed: 33152939]
Sci RepTert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats[33750916]
J Cell SciLocal DNA synthesis is critical for DNA repair during oocyte maturation[34415018]
Stem Cell Rev RepComparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic …[34859376]
Naunyn-Schmiedeberg's ArchivesInflammatory Modulation of miR-155 Inhibits Doxorubicin‑Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus …[Pubmed:35715546]
AntioxidantsTherapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model …[Pubmed:35204140]
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …[]
Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation …[]