内脂素(VF)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Visfatin (VF)
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
- 编号KSA638Hu11
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围1.23-100ng/mL
- 可适灵敏度
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 4882¥ 8136¥ 14645¥ 28476¥ 48816
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
产品图片
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对内脂素(VF)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA638Hu01 | 内脂素(VF)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA638Hu21 | 内脂素(VF)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA638Hu01 | 内脂素(VF)多克隆抗体 | WB; IHC |
| PAA638Hu08 | 内脂素(VF)多克隆抗体 | WB; IHC; ICC; IP. |
| FAA638Hu01 | 抗内脂素(VF)多克隆抗体 | Flow cytometry. |
| MAA638Hu22 | 内脂素(VF)单克隆抗体 | WB |
| MAA638Hu27 | 内脂素(VF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA638Hu29 | 内脂素(VF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA638Hu21 | 内脂素(VF)单克隆抗体 | WB |
| MAA638Hu23 | 内脂素(VF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA638Hu24 | 内脂素(VF)单克隆抗体 | WB; IHC |
| MAA638Hu25 | 内脂素(VF)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA638Hu26 | 内脂素(VF)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA638Hu04 | 抗内脂素(VF)单克隆抗体 | Flow cytometry. |
| FAA638Hu02 | 抗内脂素(VF)单克隆抗体 | Flow cytometry. |
| FAA638Hu06 | 抗内脂素(VF)单克隆抗体 | Flow cytometry. |
| FAA638Hu08 | 抗内脂素(VF)单克隆抗体 | Flow cytometry. |
| SEA638Hu | 内脂素(VF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA638Hu | 内脂素(VF)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA638Hu | 内脂素(VF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA638Hu | 内脂素(VF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA638Hu11 | 内脂素(VF)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Brazilian Journal of Biomoricity | EFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MEN[Brjb: source] |
| Diabetes Research and Clinical Practice | The relationship between visfatin and HOMA-IR in hypertensive patients, and the effect of antihypertensive drugs on visfatin and HOMA-IR in hypertensive patients with insulin resistance[ScienceDirect: S0168822711002956] |
| J Physiol Pharmacol. | Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat.[Pubmed: 24304571] |
| Endocr J. | CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes[Pubmed:25168658] |
| Exp Ther Med. | Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndrome[Pubmed:Pmc3991522] |
| Eur J Cancer Prev. | Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization.[Pubmed:25055182] |
| Genome | Visfatin −948G/T and resistin −420C/Gpolymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases[Nrcresearchpress:Source] |
| PLOS ONE | Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug[PubMed: 26262991] |
| Alcoholism and Drug Addiction | Changes in concentration of visfatin during four weeks of inpatient treatment of alcohol dependent males[Science: Article] |
| Cytokine | Variation in blood levels of hormones in obese patients following weight reduction induced by endoscopic and surgical bariatric therapies[PubMed: 26539806] |
| Genome | Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 2 Diabetic Patients With and Without Complications[PubMed: 25120107] |
| Acta Histochemica | Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines[science:S0065128116301234] |
| Endocrine, Metabolic & Immune Disorders-Drug Targets | Changes in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy.[pubmed:28699485] |
| Endocrine Connections | Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery[Pubmed:30012648] |
| Bioscience Reports | Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats[Pubmed:29784871] |
| Contraception | Hyperinsulinemic-euglycemic clamp over the first year of use of depot-medroxyprogesterone acetate as a contraceptive[Pubmed:29665358] |
| Cranio - Journal of Craniomandibular Practice | Analysis of synovial fluid visfatin level in temporomandibular joint disorders[Pubmed:29385907] |
| Nutrire | Resistin and visfatin concentrations are related to central obesity and inflammation in Brazilian children[10.1186:s41110-018-0060-7] |
| DNA and Cell Biology | Transcriptional Regulation of NAMPT Gene by Glycogen Synthase Kinase 3β in Goat Adipocytes[Pubmed: 30615481] |
| Jundishapur Journal of Chronic Disease Care | The Effect of 8 Weeks Aquatic Exercise Program on Plasma Visfatin Level and Some Blood Factors in Obese Men[] |
| Biomarkers in Heart Failure and Associated Diseases | Adipokines as Biomarkers in Health and Disease[] |
| Journal of Translational Medicine | Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling pattern[Pubmed: 31533725] |
| clinical neurology and neurosurgery | Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels[Pubmed: 32311618] |
| The Horizon of Medical Sciences | Effects of 10-Week Concurrent Training on Insulin Resistance and the Serum Levels of Vaspin and Visfatin in Overweight Females[] |
| EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY | Different Ratios of Corn and Coconut Oil Blends in High©\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats[] |